氢醌对TK6细胞周期的影响及其相关的调控机制探讨

Effects of Hydroquinone on TK6 Cell Cycle and Related Regulatory Mechanism

[目的]探讨氢醌(hydroquinone,HQ)对细胞周期的影响及其相关的调控机制。[方法]磷酸盐缓冲液(PBS)处理人类淋巴母细胞(TK6细胞)为对照组,20.0μmol/L HQ为处理组,48 h后通过碘化丙啶(PI)标记的流式细胞术检测细胞周期分布情况,用实时荧光定量聚合酶链反应(qRT-PCR)检测PTEN(抑癌基因)、p53基因mRNA表达的改变,流式细胞荧光标记法检测Rb蛋白的表达。[结果]与对照组比较,处理组G1期细胞比例明显增加,S期细胞比例明显减少,差异均有统计学意义(P<0.05);HQ作用于TK6细胞后上调了Rb的表达,下调了p53的表达,其差异均有统计学意义(P<0.05),而对PTEN的表达没有明显影响。[结论]HQ可能通过上调Rb的表达使细胞阻滞于G1期。

[Objective] To investigate the effects of hydroquinone （HQ） on cell cycle and related regulatory mechanism in TK6 human lymphoblasts cells. [Methods] Phosphate buffered solution （PBS）-treated TK6 cells were selected as the control group, and 20 μmol/L HQ-treated same line cells as the HQ-treated group. The distribution of cell cycle was determined with a flow cytometry kit using propidium iodide （PI） staining, the mRNA expressions of PTEN and p53 were quantified by reverse transcription real-time polymerase chain reaction assay, and Rb protein expression was measured by flow cytometry. [Results] Compared with the control group, the proportion of G1-phase cells in the HQ-treated group was increased, while the proportion of S-phase cells was decreased, and significant differences in the above indicators were observed between the two groups （P 〈 0.05）. Up-regulated expression of Rb protein and down-regulated expression of p53 mRNA were observed in the HQ-treated group （P 〈 0.05）, but no significant effect on the expression of PTEN mRNA was observed. [Conclusion] HQ may arrest TK6 cells at G1 phase by raising the expression of Rb protein.