干扰素α-2b对早孕合并细菌性阴道病绒毛血管超微结构及血管内皮生长因子表达的影响

The effect of interferon α-2b on villous vascular ultrastructure and expressing levels of VEGF in early pregnancy women with bacterial vaginal disease

目的探讨干扰素α-2b对早孕合并细菌性阴道病(bacterial vaginal disease,BV)绒毛血管超微结构及滋养细胞血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响。方法将泸州医学院附属医院门诊就诊的早孕合并BV要求终止妊娠妇女25例作为治疗组,予以重组人干扰素α-2b栓10万IU/d治疗共5 d;选取同期正常早孕妇女30例作为对照组。评价干扰素α-2b治疗的临床疗效;并应用透射电镜观察绒毛血管超微结构及免疫组化链酶素抗生物蛋白—过氧化物酶连接(streptavidinperosidase,SP)法检测绒毛滋养细胞VEGF的表达水平。结果治疗组临床疗效评价有效率为84%;透射电镜观察到治疗组绒毛血管超微结构改变较对照组无明显差别(P>0.05);两组滋养细胞VEGF的表达水平差异无统计学意义(P>0.05)。结论干扰素α-2b治疗早孕合并BV有效,未发生绒毛血管的异常改变。

Objective To study the effect of interferon α - 2b on villous vascular uhrastructure and expressing levels of vascular endothelial growth factor（VEGF） in trophocytes in early pregnancy women with bacterial vaginal diseases（BV）. Methods The early pregnant women with BV in 25 cases who wanted to terminate pregnancy with artificial abortion were in the study group in 5 days which were treated with rhINFα - 2b. Another 30 pregnant women were in the control group without any genital tract infectious diseases. The levels of VEGF in trophocytes were detected by immunohistochemical techniques--the method of streptavidin - perosidase （SP） and the ultrastructural changes in villose blood vessels with transmission electron microscopy were observed. The clinical curative effect of rhINFα -2h in treated group was assessed. Results The clinical curative efficiency of study group was 84%. No apparent difference of ultrastruetural changes in villose blood vessels between the two groups. No statistical significance about the levels of VEGF between the two groups in trophocytes （ P 〉 0. 05 ）. Conclusion The formation of villose blood vessels would not change apparently treated efficiently with rhINFα -2b.