用于昆虫外激素研究的无分流进样及弹性毛细柱气相色谱分离几何异构体方法初探

Gas Chromatography Separation of Geometrical Isomers of Insect Sex Pheromones Using a Fused Silica Capillary Column and Splitless Injection

报导用无分流进样及毛细柱气相色谱分离昆虫外激素组分中几何异构体的初步结果。用合成的马尾松毛虫雌性引诱外激素三个组分顺 5,反 7-十二碳二烯醇 (Z - 5,E - 7-DDOL) ;顺 5,反 7-十二碳二烯乙酸酯 (Z - 5,E - 7-DDA) ;顺 5,反 7-十二碳二烯丙酸酯 (Z -5,E - 7-DDP)及它们的几何异构体作为标准化合物来探索分离进样方法。用非极性的OV -1 0 1熔融硅弹性毛细柱可分离这三个组分的顺反与顺顺、反反异构体。用极性PEG - 2 0M弹性毛细柱又可将顺反及反顺异构体分离。

Gas chromatography (GC) and gas chromatography—mass spectrometry(GC\|MS)are the optimal method for separation and structural identifications of insect pheromones. However, because MS cannot distinguish geometric isomers, they must be separated before MS identification.This study used splitless injections and fused silical capillary column to separate synthetic compounds of the female pine\|caterpillar moth(Dendrolimus punctatus walker)sex pheromones Z\|5,E\|7\|dodecadienol(Z\|5,E\|7\|DDOL),Z\|5,E\|7\|dodecadienyl acetate(Z\|5,E\|7\|DDA)and Z\|5,E\|7\|dodecadienyl propylester (Z\|5,E\|7\|DDP)and their geometrical isomers. A total of 12 geometrical isomers of these three components are well separated using the conditions established in this study, except Z\|5,E\|7\|DDA and E\|5,Z\|7\|DDA were not separated.