人神经节苷脂诱导分化相关蛋白cDNA的克隆、染色体定位和初步功能研究

Cloning, Mapping and Characterization of a Novel Human Ganglioside-Induced Gene

通过构建、筛选人 18周胎脑cDNA文库 ,克隆到一条与神经节苷脂诱导分化相关蛋白高度同源的新基因 .经HUGO/GDB人类基因命名委员会的同意命名为GDAP1L1.进行新基因的全序列测定、RH定位分析、Blast分析及生物学信息分析 .Northern杂交提示GDAP1L1基因在胎脑中高度表达 ,但在成人脑组织中低表达 .新的神经节苷脂诱导分化相关蛋白基因的表达和功能研究初步提示 :全长新神经节苷脂诱导分化相关基因核苷酸序列长 116 3bp ,RH定位分析新基因定位在染色体 2 0 q12区 ,BLASTN ,BLASTP ,TBLASTN分析新基因的蛋白质序列与人和鼠“神经节苷脂诱导分化相关蛋白 1”有 5 8%的同源性 ,而与人的另一条“类似神经节苷脂诱导分化相关蛋白 1”的部分蛋白序列 (4 7～ 2 5 3aa)的同源性达 10 0 % ,新基因蛋白在 3′ ,5′端分别多出 46aa和 114aa的长度 ,生物学信息分析证实神经节苷脂诱导分化相关基因与神经营养及与细胞凋亡有密切关系 ,全长新神经节苷脂诱导分化相关基因是一个与神经营养与发育及细胞周期调控、信号传导有关的基因 ,可能在肿瘤的发生中具有重要作用 ,其功能的进一步研究将为肿瘤机理的阐明提供思路 .

Through screening a human fetal brain cDNA library, a cDNA similar to ganglioside induced gene, was isolated. Northern analysis revealed a 1.1 kb transcript highly expressed in fetal brain presented at lower level in adult brain. The novel ganglioside induced gene was localized to chromosome 10q12 by using radiation hybrid G3 panels. Blast and information of the novel ganglioside induced gene were analysed. The novel ganglioside induced cDNA is 1 163 bp . Bioinformatical analysis of those genes has been performed. The novel ganglioside induced cDNA was associated with neurotrophic development and regulation of cell cycle and signal transduction. It is interesting and impoptant that the novel cDNA may play a critical role in carcinogenesis. Further analysis the function of novel ganglioside induced cDNA would be helpful to understand the molecular mechanism of malignant carcinoma.