人原肌球蛋白结合蛋白3(TMOD3)cDNA的克隆及功能初步分析

Cloning and Functional Analysis of Human Tropomodulin 3 Gene

从人的胎脑cDNA文库中克隆到 1条人原肌球蛋白结合蛋白 3(TMOD3)基因 .该基因cDNA序列长140 2bp ,可以编码 35 2个氨基酸残基组成的蛋白 ,与大鼠、果蝇和线虫的原肌球结合蛋白同源性分别为 82 % ,41%和 35 % .TMOD3基因定位在人 15q2 1.1 q2 1.2 ,位于遗传位标D15S146和D15S117之间 .采用基因芯片杂交的方法研究其表达谱情况 ,发现该基因在多种组织和细胞中均表达 .

A human tropomodlin gene ( TMOD 3) was cloned from a fetal brain cDNA library. The full length cDNA was 1 042 bp with an open reading frame encoding a 352 amino acid protein. The deduced protein shows 82 %, 41 % and 35 % identity to tropomodulin of rat, Drosophila and C. elegans , respectively. The TMOD 3 gene was localized to human chromosome 15q21.1 q21.2 between markers D15S146 and D15S117. The tissue distribution of TMOD 3 gene was determined by DNA microarray.