甜叶菊叶片离体培养及试管无性系的建立

The Tissue Culture and Plantlet Regeneration in Vitro of Stevia Rebaudiana Bertoni

研究了离体条件下甜叶菊 (Stivia rebaudiana Bertoni)叶片愈伤组织诱导和植株再生技术 .离体培养以 MS为基本培养基并附加 30 0 mg/L水解酪蛋白 .离体叶片在 BA0 .5mg/L和NAA0 .5mg/L的培养基上诱导形成愈伤组织 .在 BA0 .5mg/L和 NAA0 .0 5mg/L的培养基上可诱导愈伤组织分化不定芽 ,分化频率为 1 0 0 % .不定芽在 White基本培养基并附加 NAA0 .0 1 mg/L的培养基上诱导生根 ,生根率可达 1 0 0 % .炼苗后移栽 ,成活率达 95%以上 .

The research of callus induction and plantlet regeneration in vitro of Stevia rebaudiana Bertoni was carried out using the tender leaves and MS medium as the basic medium. The effects of plant growth regulators on the plantlet regeneration were compared. Callus can be induced from the excised leaves that were cultured on the basic medium supplemented with 0.5 mg/L BA and 0.5 mg/L NAA. For the adventitious bud regeneration of the callus, supplement with 0.5 mg/L BA and 0.05 mg/L NAA was appropriate, giving a regeneration frequency of 100%. Rooting occurred on the White medium supplemented with 0.01 mg/L NAA. The rooting rate could reach up to 100%. The technique of somatic cell cloning of Stevia rebaudiana Bertoni was established in vitro.