蚕蛹粗提浸液致敏小鼠哮喘模型的建立

Establishment of an asthmatic mouse model sensitized by silkworm chrysalis crude extraction

目的建立蚕蛹粗提浸液致敏Balb/c小鼠哮喘模型,检测分析蚕蛹粗蛋白致敏性。方法以Balb/c小鼠为模型实验动物,分为阴性组、阳性组。阴性组腹腔皮下注射生理盐水;阳性组腹腔注射蚕蛹粗提浸液致敏,连续3次,每次间隔1周。然后滴鼻激发1周每天1次。末次激发后24 h内进行气道高检测,收集支气管肺泡灌洗液(BALF)并对细胞进行分类、计数。检测特异性抗体(IgE)、细胞因子(IL-4、IL-10、INF-γ)以及肺组织病理变化等指标对模型的构建进行评价。结果阳性组的小鼠肺部病理改变明显,呈明显炎症病理改变,灌洗液中细胞计数明显升高,嗜酸性粒细胞明显升高(P<0.01),灌洗液IL-4、IL-10升高,INF-γ降低;阴性组各项指标均无升高。结论蚕蛹粗提浸液能使Balb/c小鼠发生致敏性哮喘,且致敏性强,建模成功,为以后相关过敏疾病防治奠定科研实验基础。

To develop an animal model of asthma in Balb/c mice sensitized by silkworm chrysalis crude extraction, and to assay the allergenicity of silkworm chrysalis crude extraction, Balb/c mice as the experimental animal model were divided into negative group and positive group. Negative group accepted intraperitoneal injection of physiological saline, while positive group accepted intraperitoneal injection of silkworm chrysalis crude extract for sensitization, continuous 3 times, with an interval of 1 week. And then, we detected airway hyperresponsiveness within 24 hours after the last inspire, and collected bronchoalveolar lavage fluid （BALF） for cell sorting and counting. Furthermore, we assayed the levels of specific antibodies （IgE） and cytokines （IL-4, IL-10, the INF-＇,/） in serum and the pathological changes of the uhrastructure of lung tissue, for evaluating the model building. Result indicated that the positive group mice demonstrated pulmonary pathological changes （obvious inflammatory pathological changes）; furthermore, in lavage fluid of positive mice, the cell counting, eosinophilic granulocyte level （P〈 0.01）, and the IL-4 and IL-10 levels were all increased, but INF-γ level reduced. While the indicators of negative group had no changes. In conclusion, all results suggested that silkworm chrysalis crude extract can cause Balb/c mice sensitization asthma, and the sensitization is strong; the successfully constructed model can provide scientific experimental basis for the control and prevention of allergic disease.