肥大细胞及蛋白酶激活受体-2在肠易激综合征中的作用

Role of mast cells and protease activated receptor-2 in irritable bowel syndrome

目的:探讨肥大细胞及蛋白酶激活受体-2(protease activated receptor-2,PAR-2)在肠易激综合征(irritable bowel syndrome,IBS)中的作用.方法:2012-01/2012-12自南京医科大学附属南京医院消化内科门诊随机选取腹泻型IBS患者60例及正常者30例,采用免疫组织化学检测各组肥大细胞、PAR-2蛋白的表达强度;采用Western blot检测各组Occludin的表达量.结果:免疫组织化学法结果显示IBS-D组与对照组相比,肥大细胞表达明显增多(5.20±2.78vs 1.40±0.55,P<0.05);PAR-2蛋白的表达明显增多(3.20±1.64 vs 1.20±0.45,P<0.05);Western blot结果示IBS-D组Occludin蛋白的表达明显低于正常组(2108.33±59.58 vs 3113.00±77.74,P<0.01).结论:本研究提示IBS-D患者肥大细胞过表达、激活后可刺激PAR-2活化,从而进一步损伤细胞间紧密连接.

AIM： To investigate the role of mast cells and protease activated receptor-2 （PAR-2） in irritable bowel syndrome （IBS）. METHODS： A total of 90 subjects were divided into a diarrhea-predominant irritable bowel syndrome （IBS-D） group （n = 60） and a normal control group （n = 30）, all of them were selected from digestive outpatient department of Nanjing Hospital Affiliated to Nanjing Medical Univer- sity from January 2012 to December 2012. The expression of mast cells and PAR-2 was detected by immunohistochemistry, and the expression of Occludin was detected by Western blot. RESULTS： Immunohistochemistry analysis showed that compared with the normal control group, the expression of both mast cells and PAR-2 was significantly higher in the IBS-D group （5.20 ±2.78 vs 1.40 ±0.55, P 〈 0.05; 3.20 ±1.64 vs 1.20± 0.45, P 〈 0.05）. Western blot analy- sis indicated that the expression of Occludin decreased significantly in the IBS-D group com- pared with the normal control group （2108.33± 59.58 vs 3113.00 ± 77.74, P 〈 0.01）. CONCLUSION： Our study suggests that in IBS-D subjects, overexpressed and activated mast cells may stimulate PAR-2 and lead to the damage of intercellular tight junctions.