EphB1在卵巢浆液性腺癌中的表达及临床意义

Expression and significance of EphB1 protein in ovarian serous adenocarcinoma

目的探讨受体酪氨酸激酶EphB1在卵巢浆液性腺癌中的表达水平及其与临床病理特征和预后的关系。方法采用免疫组化染色法检测EphB1在74例卵巢浆液性腺癌组织中的表达情况,将免疫组化检测结果分为低表达(<6分)和高表达(≥6分);分析其表达与临床病理特征和预后的关系。结果 74例卵巢浆液性腺癌组织中EphB1阳性表达67例(90.5%),阴性表达7例(9.5%);高表达42例(56.8%),低表达32例(43.2%)。EphB1表达在不同WHO分级、肿瘤直径、MDACC分级、转移情况及Ki-67指数间的差异有统计学意义(P<0.05);在不同年龄、发生部位、临床分期和复发情况间的差异无统计学意义(P>0.05)。EphB1高表达患者的3、5年生存率分别为71.2%和65.5%,低表达者均为68.3%,差异均无统计学意义(P<0.05);当随访时间>6年,EphB1低表达者的生存率较高表达者有下降趋势。结论 EphB1在卵巢浆液性腺癌组织中阳性表达,且以高表达为主,与卵巢癌的发展及分化程度有关,有可能成为卵巢浆液性腺癌预后的新标志物。

Objective To explore the protein expression levels of EphB1 in ovarian serous adenoearcinoma and analyze its correlation with clinicopathological characteristics and prognosis. Methods The protein expression levels of EphB1 in 74 ovarian serous adenocareinoma tissues were detected by immunohistochemieal staining. The results were divided into low-level expression group （〈6） and high-level expression group（≥6） according to the immunohistochemical staining counting method. The relationship between expression of EphBl and clinieopathological characteristics and prognosis was investigated. Results Out of 74 tissue samples of ovarian serous adenocareinoma, 67 cases were of positive expression（90. 5%） and 7 cases were of negative expression（9. 5%） , while low-level expression was in 32 cases（43.2% ） and high-level expression in 42 cases （56. 8% ）. There were significant con＇elation be- tween the expression of EphBl and WHO grading, tumor diameter, metastasis, M DACC grade and Ki-67 index（P〈0. 05）. No signifi- cant correlation existed between the expression of EphB 1 and age, location, clinical stage and recurrence （P〉0. 05）.There were no sig- nificant differences on 3-and 5-year survival rates between low-level expression group （ both 68.3%） and high-level expression group （ 71.2% and 65.5%） , but a downward trend was observed in low-level expression group compared with high-level expression group followed up over 6 years. Conclusion The EphB1 is positively expressed in ovarian serous adenocarcinoma tissue, mainly in high- level expression pattern. The protein expression of EphB1 is correlated with development and degree of differentiation in ovarian cancer, suggesting a role as a new indicator of prognosis in ovarian serous adenocarcinoma.