169例国人结直肠癌患者BRAF基因的突变状态分析

Analysis of BRAF gene mutation status in 169 Chinese colorectal cancer patients

目的探讨国人结直肠癌患者鼠类肉瘤病毒癌基因同源物Bl（BRAF）基因的突变状态，以期能为针对表皮生长因子受体（EGFR）的靶向治疗提供指导。方法采用PCR测序法检测169例结直肠癌组织BRAF基因的突变情况。结果在169例结直肠癌组织中，BRAF基因在第600位密码子（V600E）发生突变，突变频率为5．3％。BRAF基因在84例男性结直肠癌患者中的突变频率为7．1％，在85例女性患者中为3．5％，差异无统计学意义（P=0．329）。在17例结直肠癌年轻患者（25～44岁）中未检测到BRAF基因突变，在67例中年患者（45～59岁）中BRAF基因的突变频率为3．0％，在85例老年患者（60～90岁）中为8．2％，三者之间差异无统计学意义（P=0．211）。结论BRAF基因的突变频率与结直肠癌患者的性别和年龄无关。

Objective To analyze the mutation status of v-raf routine sarcoma viral oncogene homolog BI （BRAF） gene in Chinese colorectal cancer（CRC） patients, and to promote anti-epidemal growth factor receptor（EGFR） therapy. Methods The muta- tion of BRAF gene in 169 CRC tissues were detected by PCR direct sequencing. Results The mutation was found at the 600th codon （V60OE） of BRAF gene, and the mutation frequency was 5.3% in 169 CRC tissues. Moreover, the mutation frequency of BRAF gene was 7. 1% in 84 male and 3.5% in 85 female CRC without statistical significance（P= 0. 329）. No mutation was found in 17 CRC youth patients（25-44 years old）, the mutation frequency was 3.0% in 67 CRC middle age patients（45-59 years old）, and 8.2% in 85 CRC elder patients （60-90 years old） without statistical significance （P = 0. 211 ）. Conclusion The mutation frequency of BRAF gene in CRC patients is not related to gender and age.