SU11274对人结肠癌LOVO细胞裸鼠移植瘤的抑制作用

Inhibitive effect of SU11274 on human colon cancer( LOVO cell line) transplanted in nude mice

目的观察小分子酪氨酸激酶抑制剂SU11274对人结肠癌LOVO细胞裸鼠移植瘤的抑制作用。方法建立人结肠癌LOVO细胞裸鼠移植瘤模型,18只裸鼠随机分成对照组、大剂量组和小剂量组,每组6只。采用腹腔注射,对照组给予灭菌生理盐水,大剂量组和小剂量组分别给予0.09 mg/kg、0.022 5 mg/kg剂量的SU11274(SU11274溶解于1%DMSO中),观察各组裸鼠肿瘤的生长情况,称体质量并计算抑瘤率。免疫组化法检测c-Met的表达,并进行HE染色。结果 SU11274大剂量组肿瘤体积较对照组明显减小,差异有统计学意义(P<0.05),SU11274小剂量组肿瘤体积较对照组差异无统计学意义(P>0.05);SU11274大剂量组对人结肠癌LOVO细胞裸鼠移植瘤生长具有明显的抑制作用,抑瘤率为56.25%,SU11274小剂量组裸鼠肿瘤生长的抑制作用不明显,抑瘤率为25.00%;SU11274大剂量组c-Met阳性表达率为(45.83±8.61)%,明显低于对照组(66.67±16.63)%(P<0.05),SU11274小剂量组中c-Met阳性表达率为(63.33±8.16)%,与对照组相比差异无统计学意义(P>0.05)。结论 SU11274可显著抑制人结肠癌裸鼠移植瘤的生长。

Objective To investigate the inhibitive effect of tyrosine kinase inhibitor SU11274 on the growth of human colon cancer （ LOVO cell line） using a nude mice transplanted model. Methods LOVO cancer cells were xenografted into 18 nude mice, which were then randomly divided into 3 groups： control group, high dose SU11274 treatment group （0.09 mg/kg） and low dose SUl1274 treatment group （0.022 5 mg/kg）. Tumor growth was observed, the mice were weighed, and the tumor inhibition rate was calculated. The expression of c-Met was detected with immunohistochemical method, and HE staining was conducted. Results The mean tumor volume in the high dose SU11274 treatment group was significantly lower than that in the control group （P 〈0.05 ）. The tumor inhibition rate of the high dose SUl1274 treatment group was much higher than that of the control group （56.25% vs 25.00%, P 〈0.05）. Furthermore, the positiverate of c-Met expression was （45.83 ＋ 8.61 ） % in the high dose SUl1274 treatment group, and （66.67 ＋ 16.63 ） % in the control group, with significant difference between the two groups （P 〈0.05）. Conclusion SU11274 could in- hibit the human colon cancer cell transplanted tumor in nude mice.