基因重组人三叶因子3治疗烧伤小鼠肠黏膜损伤的实验研究

Treatment of burn-induced intestinal injury in mice with recombinant hTFF3

目的观察基因重组hTFF3对严重烧伤所致肠黏膜损害的治疗作用。方法采用30%体表面积Ⅲ度烧伤小鼠模型,随机分成对照组、烧伤组和烧伤后hTFF3治疗(TFF3)组,TFF3组伤后1 h开始hTFF3灌胃,剂量为1 mg/kg,烧伤组和对照组灌胃等量生理盐水,各组每天灌胃1次,连续5 d。观察烧伤后肠组织病理学改变,肠黏膜肠绒毛高度、隐窝深度及病死率。结果烧伤小鼠每天给予1 mg/kg的hTFF3灌胃,显著降低肠黏膜受损程度,肠黏膜病理改变明显减轻,烧伤组以出血、坏死和溃疡为主,TFF3组以充血、水肿为主,同时肠黏膜厚度、绒毛高度、隐窝深度明显增加,小鼠5 d病死率有所下降(27.3%vs 45.6%)。结论重组hTFF3对烧伤后肠道损伤具有明显的治疗作用,能促进受损黏膜屏障的重建。

Objective To explore the effect of recombinant hTFF3 on burn-induced intestinal injury in mice. Methods Kunming mice were inflicted on the back with IlI degree bum injury on 30 % of total body surface, and then they were randomly divided into three experimental groups： sham ＋ saline group, bum ＋ saline group, and bum ＋ hTFF3 （ group TFF3）. Mice in group TFF3 were exposed to thermal injury, administered orally with hTFF3 protein （ 1 mg/kg） dissolved in saline by gastric gavage immediately after recovering from anesthesia, and continued with the same dose once a day. Mice in bum ＋ saline group received thermal injury and saline in equal volume once a day. Mice in sham ＋ saline group were exposed to 21 ~C water and saline after recovering from anesthesia. Pathomorphological changes, vil- lous height, crypt depth, and mortality rate after injury were observed. Results Gastric gavage with hTFF3 （lmg/kg） daily significantly alleviated the damage and pathological changes of intestinal mucosa. Mice in bum ＋ saline group manifested hemorrhage, necrosis and ulcer. Mice in group hTFF3 presented congestion and edema, and at the same time, the thickness of intestinal mucosa, the height of villous and depth of crypt were markedly increased, and the death rate at day 5 was reduced（27.3% vs 45.6% ）. Conclusion Recombinant hTFF3 has significant therapeutic effect on the bum-induced intestinal injury by promoting the reconstruction of injured intestinal mucosa.