温州光唇鱼线粒体基因组结构及系统发育分析

Mitochondrial genome structure of Acrossocheilus wenchowensis and phylogenetic analysis

采用已公布的鱼类线粒体基因组全序列,设计8对引物扩增、测定并注释温州光唇鱼(Acrossocheilus wenchowensis)线粒体基因组(GenBank登录号:KC_495074)。序列全长16 591 bp,包括13个蛋白质基因、22个tRNA基因、2个rRNA基因和1个非编码区,各基因的位置及组成与已公布的鲤科鱼类一致;37个基因中,1个蛋白质编码基因(ND6)和8个tRNA基因(tRNAGln、tRNAAla、tRNAAsn、tRNACys、tRNATyr、tRNASer(UCN)、tRNAGlu和tRNAPro)由L链编码,其余的均由H链编码。A、T、G、C碱基组成分别为30.92%、24.86%、16.41%、27.81%;除tRNASer(AGN)外,其它21个tRNA的二级结构均具有典型的三叶草结构;13个蛋白编码基因中,除COⅠ起始密码子为GTG外,其余均以ATG为起始密码子,而COⅡ、ND4和Cytb基因的终止密码子为不完整的T,其它10个基因均具有完整的终止密码子。利用鲤科共17属18种线粒体基因组13个蛋白质基因的氨基酸序列,从线粒体基因组水平探讨了温州光唇鱼在鲤科鱼类中的系统进化地位,为光唇鱼属乃至鲤科鱼类的系统分类学研究提供基础资料。

The complete mitochondrial genome of Acrossocheilus wenchowensis was obtained by PCR based on 8 primers, which were designed on the basis of published fish mitochondrial genome （GenBank accession number： KC_495074）. The complete mitochondrial genome was 16 591 bp in length, including 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and a non-coding control region. The organization and location of genes in the mitochondrial genome of A.wenchowensis were consistent with Cyprinidae fishes published in GenBank. Of 37 genes, 1 protein-coding gene（ND6） and 8 tRNA genes（tRNAGln, tRNAAla, tRNAAsn, tRNACys, tRNATyr, tRNASer（UCN）, tRNAGlu and tRNAPro） were encoded on the L strand, the remainders on the H strand. The nucleotide composition was 30.93% （A）, 24.86% （T）, 16.41% （G） and 27.81% （C）. Most tRNA genes could form typical secondary structures except tRNASer（AGN）. The COⅠ gene used GTG as an initiation codon, with the remaining 12 using ATG of total 13 protein genes, 10 genes had complete stop codons,and COⅡ, ND4 and Cytb had an incomplete stop codon T. The phylogenetic analysis of A.wenchowensis on Cyprinidae fishes was made from the level of mitochondrial genome based on amino acids sequence of all protein-coding genes in 18 species of 17 genera, and the complete mitochondrial genome of A.wenchowensis will help to provide basic data for the classification of Acrossocheilu and Cyprinidae fishes.