瓯江彩鲤肌间小骨的骨化模式

Ossification pattern of the intermuscular bone in Cyprinus carpio var. color

分别利用形态解剖和整体骨骼染色的方法,对瓯江彩鲤(Cyprinus carpio var.color)成鱼肌间小骨的形态、分布,以及仔、稚鱼肌间小骨的形态发生和出现进行观察。结果表明,瓯江彩鲤肌间小骨有I形、卜形、Y形、一端多叉形、两端两叉形、两端多叉形和树枝形7种类型,肌间小骨越靠前端形态越复杂。肌间小骨出现前,其他骨骼均已骨化完成,肌间小骨在32 dpf(day past fertilization)首先出现在尾部,然后往前依次出现,到53 dpf全部出现。各种复杂形态的肌间小骨均是从I形发展而来。经比较,鲤科不同亚科鱼类肌间小骨在骨化时机和骨化形态方面具有相似的形态发生规律,为今后研究肌间小骨发生的分子机制提供了形态学基础。

To further understand the development process of intermuscular bones in teleosts, a modified bone clearing and staining experiment was conducted to investigate morphological development of intermuscular bone in Cyprinus carpio var. color. It is showed that intermuscular bone has different shapes including I type, one-end-unequal-bifork type, one-end-equal-bifork type, one-end-multifork type, two-end-biofork type, two-end-multifork type and tree-branch type. Generally, the morphological complexity of most intermuscular bones in anterior body was greater than that in posterior body. The initial ossification of intermuscular bone was observed in the posterior part of the body at 32 dpf, and then each of the intermuscular bones is ossified anteriorly, and the morphological characters at 53 dpf are the same as those found in adult fish. The primary morphology of intermuscular bone is I type, and more complicated morphology of ossified intermuscular bones are gradually derived from I type during ossification. These results suggest similar ossification pattern of intermuscular bones existing in Cyprinidae, which will also be helpful for us to further understand the molecular mechanism underlying intermuscular bone development.