2012年我国部分省市规模化猪场副猪嗜血杆菌分离鉴定及菌株血清分型

Isolation,Identification and Serotyping of Haemophilus parasuis in Parts of Larg-scale Pig Farms in China 2012

为掌握2012年我国猪场副猪嗜血杆菌(Hps)感染的发病状况,在我国部分省市规模化猪场采集疑似副猪嗜血杆菌病猪的心包积液及发生纤维素性浆膜炎的肺脏等病料共168份,通过病原分离培养、形态及培养特性观察、生化试验、PCR扩增等方法进行鉴定,最终分离出51株副猪嗜血杆菌,分离率约为30.4%。对51株副猪嗜血杆菌用琼脂扩散法进行血清分型,得到4型的6株(11.8%),5型的9株(17.6%),13型9株(17.6%),12型、14型各2株,1型、6型、10型、11型各1株,未能确定血清型19株,4型、5型、13型占总比例的47.1%,占有毒力血清型(强毒和中等毒力血清型)比例80.0%,表明副猪嗜血杆菌4型、5型、13型菌株是我国的优势血清型。

In this study,168 field samples suspected with the infection of Haemophilusparasuis （Hps）were collected from several domestic large-scale pig farms in China.Among them,5 1 samples were identified as Hps by bacterial morphological analysis,biochemistry tests and PCR.The isolation rate was 30.4%.The Hps-positive isolates were subsequently serotyped by agarose diffusion test （ADT）.Thirty two isolates could be successfully serotyped,belonging to serotype 4 （6 isolates）,serotype 5 （9 isolates）,serotype 1 3 （9 isolates）,serotype 12 （2 isolates）,serotype 14 （2 isolates）,serotype 1 （1 isolate）,serotype 6 （1 isolate）, serotype 10 （1 isolate）,serotype 11 （1 isolate）.Nineteen isolates could not be determined in serotyping. Collectively,24 isolates belonged to serotype 4,5 and 13,which was as high as 47.1% of the total isolates and up to 80% of the virulent isolates.These results suggest that Hps was one of the most common patho-gens in pigs in China,and seroypes 4,5 and 13 of Hps were the most prevalent ones.