拓扑替康对人肝癌BEL-7402细胞体外迁移、黏附的影响

Effect of topotecan on migration and adhesion of human hepatoma BEL-7402 cells in vitro

目的研究拓扑替康(topotecan,TPT)对人肝癌BEL-7402细胞体外迁移、黏附的影响。方法体外培养人肝癌BEL-7402细胞,分为实验组和空白组,实验组分为三个亚组,分别用0.1、0.2、0.3 mmol/L的TPT进行干预,空白组给予不含TPT的培养液。采用划痕实验和Transwell小室迁移实验检测TPT对BEL-7402细胞体外迁移能力的影响,采用黏附实验检测TPT对BEL-7402细胞黏附和异质黏附能力的影响。结果0.2、0.3 mmol/L TPT组中BEL-7402细胞的体外迁移能力受到抑制,同质黏附能力增强,而异质黏附能力减弱,差异具有统计学意义(P均<0.05)。结论拓扑替康可抑制肝癌BEL-7402细胞的迁移和异质黏附能力,增强肝癌细胞的同质黏附能力。

Objective To study the effects of topotecan （TPT） on migration and adhesion of the human hepatoma cell line BEL-7402 cells in vitro. Methods The human hepatoma cell line BEL-7402 cells were cultured in vitro, and divided into two groups： experimental group and control group. The experimental group was divided into three subgroups and treated with 0.1, 0.2 and 0.3 mmol/L TPT respectively. The control group was treated with culture medium without TPT. Effects of TPT on ability of migration of BEL-7402 cells was detected by wound-healing assay and Transwell chamber model. Effects of TPT on ability of homogeneous and heteroge- neous adhesion of BEL-7402 cells was assessed by adhesion experiments. Results The ability of migration and heterogeneous adhesion were significantly depressed in 0.2 and 0.3 mmol/L TPT group （P〈0.05）. The ability of homogeneous adhesion was effectively enchanced after treated with 0.2 and 0.3 mmol/L TPT （P〈0.05）. Conclusion TPT can depress the migratory and heterogeneous adhesion potentials, and it can enchance the homogeneous adhesion potential of the human hepatoma cell line BEL-7402 cells in vitro.