摘要
目的:建立HPLC-ELSD测定尼莫地平脂肪孔注射液中溶血磷脂酰胆碱(LPC)的含量。方法:采用Kromasil 100-5SIL(250 mm×4.6 mm,5μm)色谱柱,以甲醇-冰醋酸(500:10,三乙胺调节pH至6.0)为流动相,流速为1.0 ml·min^(-1),柱温为30℃,使用蒸发光散射检测器(雾化气:氮气,漂移管温度:60℃)。结果:样品中磷脂酰胆碱(PC)及LPC与相邻杂质峰分离效果良好;LPC定量限为218.2 ng,检测限为65.46 ng;平均回收率为100.9%,RSD为3.3%(n=9)。结论:本法简便、专属性强、准确可靠,可用于尼莫地平脂肪乳注射液中溶血磷脂酰胆碱的含量测定。
Objective： To establish an HPLC-ELSD method for the determination of lysophosphatidyleboline in nimodipine fat e- mulsion injections. Methods： A Kromasil 100-5SIL（250 mm×215;4.6 mm,5 μm）column was used with methanol-acetic acid（ 500： 10, adjusting pH to 6.0 with triethylamine） as the mobile phase. The flow rate was 1.0 ml·min^-1 , the column temperature was set at 30℃ and the detector was an ELSD with nitrogen as the carrier and drift tube temperature of 60℃. Resu.lts： Phosphatidylcholine and lysophosphatidylcholine were completely separated from the other components in the fat emulsion injections. The quantitative limit and detection limit of lysophosphatidylcholine was 218.2ng and 65.46ng, respectively. The average recovery was 100.9% （RSD = 3.3%, n = 9）. Conclusion： The method is simple, specific, accurate and reliable, and can be used in the determination of lysophosphatidycho- line in nimodipine fat emulsion injections.
出处
《中国药师》
CAS
2014年第1期87-90,共4页
China Pharmacist
