HPLC法测定安神补脑液中2,3,5,4'-四羟基二苯乙烯-2-O-β-D葡萄糖苷的含量

Determination of 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-Glucoside in Anshen Bunao Liquids by HPLC

目的:建立安神补脑液中2,3,5,4'-四羟基二苯乙烯-2-O-β-D葡萄糖苷的含量测定方法。方法:采用HPLC法,Agilent EcLipse XDB-C_(18)柱(250 mm×4.6 mm,5μm),乙腈-水(19:81)为流动相,流速为1.0 ml·min^(-1),荧光检测器,检测波长为λ_(ex)=360 nm,λ_(em)=417 nm。结果:2,3,5,4'-四羟基二苯乙烯-2-O-β-D葡萄糖苷在5.34~213.60μg·ml^(-1)范围内线性关系良好,平均回收率为100.7%,RSD为1.8%(n=9)。结论:2,3,5,4'-四羟基二苯乙烯-2-O-β-D葡萄糖苷在5.34~213.60μg·ml^(-1)范围内线性关系良好,平均回收率为100.7%,RSD为1.8%(n=9)。

Objective： To establish the determination method of 2,3,5,4＇-tetrahydroxystilbene-2-O-β-D-glucoside in Anshen Bu- nao liquids. Methods： An HPLC method was used, the chromatographic condition consisted of an Agilent EcLipse XDB-C18 （250 mm× 4.6 mm,5 μm） column with acetonitrile-water （ 19：81 ） as the mobile phase at a flow rate of 1.0 ml·min^-1 , the absorption detec- tion wavelength was at 360 nm and the emanation detection wavelength was at 417 nm. Results： 2,3,5,4＇-Tetrahydroxystilbene-2-O-β- D-glucoside showed a linear relationship within the range of 5.34-213.60 μg·ml^-1 , and the average recovery was 100.7% with RSD of 1.8% （n = 9）. Conclusion： The method is simple and accurate with high sensitivity and good reproducibility, and can be used in the quality control of Anshen Bunao liquids.