巴中产栀子解热抗炎药理效应研究

Antipyretic and Anti-inflammatory Effects of Gardenia from Bazhong

目的:探索巴中产栀子对内毒素所致家兔发热模型的解热药理效应及其抑制0.6%冰醋酸所致小鼠腹腔毛细血管通透性增加的抗炎药理效应。方法:针对家兔耳缘静脉注射内毒素生理盐水溶液制备发热模型与0.6%冰醋酸溶液所致小鼠腹腔毛细管通透性增加的炎症模型,分别灌胃给予栀子水溶性有效部位、70%醇溶性有效部位,测定家兔体温、小白鼠腹腔液吸光度值,观察不同产地栀子的解热抗炎作用。结果:巴中产栀子提取水溶性有效部位对发热家兔的降温效果优于江西、湖北、江津产栀子(P<0.01);巴中产栀子提取醇溶性有效部位对发热家兔的降温效果明显优于江津和江西栀子70%醇溶性有效部位(P<0.05或0.01),与湖北产栀子比较有降低趋势,但差异无统计学意义(P>0.05)。巴中产栀子有效性部位抑制0.6%冰醋酸所致小鼠腹腔毛细血管通透性增加的效果与江西、湖北、江津产栀子相比,差异无统计学意义(P>0.05)。结论:巴中产栀子在解热方面的品质更优,疗效更佳。

Objective： To explore the antipyretic effect on rabbit fever induced by endotoxin and the anti-inflammatory effect on the increase of mouse abdominal cavity capillary permeability caused by 0.6% glacial acetic acid of Bazhong gardenia. Methods： The rabbit fever model was made by ear margin vein injection of endotoxin in physiological saline solution, and the mouse inflammation model of abdominal cavity capillary permeability increase was established by 016% glacial acetic acid solution. The animals were respectively intragastrically administrated with gardenia water-soluble effective parts and 70% alcohol-soluble effective parts. The rabbit body temperature and mouse celiac fluid absorbance values were detected. The effects of gardenia from different habitats were studied and compared. Results： The antipyretic effect of water-soluble effective parts from Bazhong gardenia extract was better than that of gardenia extract from Jiangxi, Hubei and Jiangjin （ P 〈 0.01 ）, and that of alcohol-soluble effective parts from Bazhong gardenia extract was better than that of gardenia extract from Jiangjin and Jiangxi （ P 〈 0.05 or 0.01 ）. Although compared with that of Hubei gardenia, the antipyretic effect of alcohol-soluble effective parts from Bazhong gardenia extract was weaker than that of gardenia extract from Hubei, the difference was not significant （ P 〉 0.05 ）. Bazhong gardenia could inhibit the increase of abdominal blood capillary permeability in mouse inflammation model, and the effect was better than the other gardenia, however without significant difference （ P 〉 0.05 ）. Conclusion： Bazhong gardenia shows better antipyretic effect compared with that from other habitats.