固相萃取-高效液相色谱法检测中成药中人工合成色素

Detection of Synthetic Pigments in Chinese Patent Drugs by Solid Phase Extraction-HPLC

目的：建立检测中成药中人工合成色素的固相萃取-高效液相色谱（SPE-HPLC）分析方法。方法：样品利用聚酰胺固相萃取柱净化和富集后进行HPLC分析。采用资生堂C18色谱柱，以甲醇和0.02mol·L-1醋酸铵为流动相进行梯度洗脱，检测波长：508nm，柱温：30℃，进样量：10μl。结果：合成色素线性范围均为0.005～0.200μg（r=0.9999），回收率分别为97.6%（RSD=1.3%,n=6）、97.0%（RSD=1.4%,n=6）和99.5%（RSD=1.4%,n=6）。结论：该方法操作简单，结果准确可靠，重复性好，适用于中成药中人工合成色素的检测。

Objective： To establish a method for detecting synthetic pigments in Chinese patent drugs by solid phase extraction-HPLC（SPE-HPLC）. Methods： Purified with SPE and concentrated, the sample was separated on a SHISEIDO C18 column with 0.02 mol·L-1 ammonium acetate and methanol mixed solution as the mobile phase with gradient elution at the flow rate of 1.0 ml·min-1, the detection wavelength of 508nm, column temperature of 30℃，and the injection volume of 10μl. Results：The linear range of the synthetic pigments was 0.005-0.200 μg（r=0.999 9）, and the average recovery was 97.6%（RSD=1.3%,n=6），97.0%（RSD=1.4%,n=6） and 99.5%（RSD=1.4%,n=6）, respectively. Conclusion：The proposed method is simple, reliable and reproducible, and especially suitable for the detection of synthetic pigments in Chinese patent drugs.