摘要
目的：研究黄芩防晒成分最佳提取工艺。方法：采用紫外分光光度法检测黄芩提取液在200—400nm各区的紫外吸收率，采用高效液相色谱法测定黄芩苷含量。分别以黄芩提取液紫外吸收率，黄芩浸膏得率，黄芩苷提取量为指标，采用单因素考察和正交试验，确定黄芩防晒成分提取工艺。结果：黄芩提取液的最佳提取工艺为：提取乙醇浓度60％，料液比1：40，超声时间40min，超声温度60℃。结论：黄芩提取液在紫外线中波范围有很强的吸收，具很好的防晒作用。超声波提取法简单，合理，可行，可用于黄芩防晒成分的提取。
Objective： To optimize the extraction technology of radix scutellariae. Methods： The extraetiou of radix seutellariae was scanned by uhraviolet, speetrophotometry from 200 to 400nm. The content of baicaliu was determined by HPLC. The uhraviolet absorption, baicalin content and extraction rate were used as the indices, and the optimal extraction conditions were investigated by single factor experiments and orthogonal design tests. Results： The optimal extraction conditions were as follows： the ethanol concentration was 60% , the solid-liquid ratio was 1 ： 40, ultrasound extraction time and temperature was 40 min and 60℃ , respectively. Conclusion： The extraction of radix scutellariae has good sunscreen with promising ultraviolet absorption in UVB. Ultrasound extraction has high extraction yield with short time, which can be used to extract sun-screening ennstituents from radix scutellariae.
出处
《中国药师》
CAS
2014年第2期210-214,共5页
China Pharmacist
关键词
黄芩
黄芩苷
紫外辐射
正交试验
Radix scmellariae
Baicalin
Uhraviolet radiation（UVR）
Orthogonal design
