摘要
目的:建立一种测定长春西汀注射液中4种有关物质含量的方法。方法:采用Kromasil C18(150 mm×4.6 mm,5μm)色谱柱;以0.2 mol·L-1醋酸铵溶液-乙腈(40∶60)为流动相,检测波长为280 nm,流速为1.0 ml·min-1,进样量为20μl。结果:注射液中长春西汀与有关物质二氢长春西汀、甲氧基长春西汀、长春胺乙酯和阿朴长春胺达到完全分离;4种有关物质均有良好线性关系,平均回收率均达99.8%以上。结论:本法灵敏,准确,专属性强,可用于长春西汀注射液中4种有关物质的检查。
Objective： To establish an HPLC method for determining the contents of related substances in vinpocetine injections. Methods： The chromatographic separation was performed on a Kromasil C18 colunm （ 150 mm ×4.6mm,5μm）. The mobile phase consisted of 0.2 mol ·L-1 ammonium acetate -acetonitrile （40： 60） , the flow rate was 1.0 ml ·min-1, the detection wavelength was 280 nm, and the injection volume was 20μl. Results： Vinpocetine and the related substances such as ethyl vincaminate, apovincamine, methoxyvinpoeetine and dihydrovinpoeetine were separated .completely. The ealibration curves of related substances showed good lineal~ ity. The average recoveries of related substances were all above 99.8%. Conclusion： The method is accurate, sensitive and speeific, and can be applied in determining the related substances in vinpocetine injections.
出处
《中国药师》
CAS
2014年第2期234-238,共5页
China Pharmacist
