HPLC法测定安神益智胶囊中丹酚酸B的含量

Determination of Salvianolic Acid B in Ansheng Yizhi Capsules by HPLC

目的：建立高效液相色谱法测定安神益智胶囊中丹酚酸B的含量。方法：色谱柱为HypersilODS2C18柱（150mm×4．6mm，5μm），流动相为甲醇：水：甲酸（40：60：1），检测波长：286nm；流速：1．0ml·min-1；进样量：10μl。结果：丹酚酸B在7．75—77．51μg·ml-1的浓度范围内线性关系良好，r=0．9996，平均回收率为98．17％，RSD为1．79％（n：6）。结论：本法简便、准确，灵敏度高，重复性好，可作为控制安神益智胶囊质量的方法。

Objective： To establish an HPLC method for the content determination of salvianolic acid B in Ansheng Yizhi capsules. Methods： A Hypersil ODS2 Cls column（ 150 mm×4.6mm,5μm）was used and methanol-water-formic acid （40： 60：1 ） was used as the mobile phase. The detection wavelength was at 286 nm. The flow rate was 1.0 ml ·min-1 and the sample size was 10μl. Results ： The calibration curve of salvianolic acid B was linear within the range 7.75-77.51 μg·ml-1 （ r = 0. 999 6）. The average recovery was 98.17% （RSD = 1.79%, n =6）. Conclusion： The method is simple, accurate and repeatable, which can be used in the quality control of Ansheng Yizhi capsules.