HPLC法测定调经助孕颗粒中马钱苷的含量

Determination of Loganin in Tiaojing Zhuyun Granules by HPLC

目的：建立调经助孕颗粒中马钱苷的含量测定方法。方法：采用高效液相色谱法，色谱柱为SinoChromODS．BP（250mm×4．6mm，5μm），流动相为乙腈-0．1％磷酸水溶液（14：86），检测波长为240nm，柱温30℃。结果：马钱苷进样量在50．40～1008．00ng范围内与峰面积有良好的线性关系（r=0．9999），平均加样回收率为99．33％，RSD为1．41％（n=6）。结论：该方法快速、准确，操作简单，专属性强，可作为调经助孕颗粒中马钱苷的含量测定方法。

Objective： To establish a determination method for loganin in Tiaojing Zhuyun granules by HPLC. Methods： HPLC was used with a SinoChrom ODS-BP （250 mm×4.6mm,5μm） as the analytical column. The mobile phase was composed of acetonitrile-0.1% phosphoric acid solution （ 14： 86）. The detection wavelength was set at 240 nm and the column temperature was 30℃. Resuits ： The calibration curve of loganin was in a good linearity over the range of 50.40-1 008.00 ng（ r = 0.999 9 ）. The average recovery was 99.33 % （RSD = 1.41%, n = 6）. Conclusion： The method is simple, feasible and reproducible, and can be used in the quality control of Tiaojing Zhuyun granules.