HBV-DNA与乙型肝炎血清标志物的相关性分析

Analysis of the Correlation between Serum Immune Markers of Hepatitis B Virus and HBV-DNA

目的:探讨乙型肝炎病毒(HBV)DNA载量与其血清标志物的相关性。方法:运用荧光定量聚合酶链反应(FQ-PCR)、酶联免疫吸附实验(ELISA)分别检测503例患者HBV-DNA载量和HBV血清标志物。根据HBV血清标志物结果分为大三阳组、小三阳组、少见模式组、抗体阳性及全阴组,比较各组间HBV-DNA的阳性率及定量值。结果:在大三阳组、小三阳组、少见模式组、抗体阳性及全阴组HBV-DNA的阳性率分别为90%、65.1%、65.2%、2.0%,HBV-DNA的定量结果 (logHBV-DNA)别为6.32±1.96、2.01±1.68、3.48±2.52(抗体阳性及全阴组阳性例数过低,不纳入统计)。大三阳组HBV-DNA的阳性率显著高于小三阳组(P<0.05),大三阳组、小三阳组HBV-DNA的阳性率与少见模式组比较,差异均无统计学意义(P>0.05),但大三阳组、小三阳组、少见模式组HBV-DNA的阳性率均显著高于抗体阳性及全阴组(P<0.01)。HBsAg、HBeAg阳性组HBV-DNA的阳性率分别显著高于HBsAg、HBeAg阴性组(P<0.01)。小三阳组、少见模式组HBV-DNA载量均显著低于大三阳组(P<0.01),少见模式组HBV-DNA载量显著高于小三阳组(P<0.05)。结论:HBV-DNA的阳性率与HBeAg、HBsAg相关;HBV-DNA载量与HBV血清标志物模式相关。

Objective：To investigate the correlation of hepatitis B virus （HBV） DNA load with its serum biomarkers. Methods： HBV DNA load and immune markers were detected in 503 case of patients by Fluorescence quantitative polymerase chain reaction （FQ-PCR）and enzyme-linked immunosorbent assay（ELISA）. On the basis of the model of hepatitis B virus immune markers, the HBsAg, HBeAg and Anti-HBc positive group, HBsAg, Anti-HBe and Anti-HBc positive group, infrequent model group, antibody positive or neg- ative group were set up. Results： The positive rate ofHBV DNA in HBsAg, HBeAg and Anti-HBc positive group, HBsAg, Anti-HBe and Anti-HBc positive group, infrequent model group, antibody positive or negative group were respectively 90%, 65.1%, 65.2%, 2.0%, and the HBV DNA quantity （IogHBV DNA） were 6.32±1,96, 2.01± 1.68, 3.48± 2.52 （We rejected to analyze the HBV DNA quantity by the course of the positive case in antibody positive or negative group were so deficient.）. The positive rate of HBV-DNA in HBsAg, HBeAg, Anti-HBc positive group was higher than that in HBsAg, Anti-HBe, Anti-HBc positive group （P〈0.05）. The positive rate of HBV-DNA in HBsAg, HBeAg, Anti-HBc positive group and HBsAg,Anti-HBe, Anti-HBc positive group with infrequent model group were compared, no statistical difference was found （P〉0.05）. But the positive rate of HBV-DNA in HBsAg, HBeAg, Anti-HBc positive group, HBsAg, Anti-HBe, Anti-HBc positive group, and infrequent model group were remarkable higher than those in the antibody posi- tive or negative group （P〈0.01）. The positive rate of HBV-DNA in HBsAg positive group and HBeAg positive group were remarkable higher than those in the HBsAg negative group and HBeAg negative group （P〈0.01）. The load of HBV-DNA in HBsAg, Anti-HBe, Anti-HBc positive group and infrequent model group were remarkable lower than that in the HBsAg, HBeAg, Anti-HBc positive group （P〈0.01）, and the load of HBV-DNA in infrequent model group was remarkable higher than that in the HBsAg, Anti-HBe, Anti-HBc positive group （P〈0.05）. Conclusion： The positive rate ofHBV DNA was related to the HBeAg and HBsAg; theHBV DNA quantity was related to the model of HBV immune markers in serum.