PDGF-A在年龄相关性白内障晶状体上皮细胞中的表达及其临床意义

Expression and Clinical Significance of PDGF-A in Epithelial Cells of Age-related Cataract Lens

目的：探讨血小板源性生长子A（Platelet derived growth factor—A，PDGF-A）在年龄相关性白内障晶状体上皮细胞中的表达及其临床意义。方法：以哈尔滨医科大学附属第四医院眼科2011年6月-2012年1月收治的72例年龄相关性白内障晶状体前囊膜作为实验组，10例无白内障发生的透明晶状体前囊膜作为正常对照组，采用sP免疫组织化学方法分别检测两组上皮细胞中PDGF—A的表达，运用图像分析软件进行量化，并采用SPSSll．0软件进行统计学分析探讨其与年龄相关性白内障·临床特征之间的关系。结果：年龄相关性白内障及透明晶状体上皮细胞中均有PDGF—A表达，且在年龄相关性白内障中表达明显高于透明对照组，差异有统计学意义（P〈0．05）。随晶状体混浊程度加重，PDGF-A强阳性表达率逐渐升高，差异有统计学意义（P〈0．05）；另外，不同年龄组白内障人群中PDGF—A表达无显著性差异（P〉0．05）。结论：①PDGF—A在年龄相关性白内障患者晶状体上皮细胞中呈高表达；②年龄相关性白内障患者中PDGF—A的表达量与晶状体的混浊程度呈正相关；（3）PDGF．A的过度表达可能在年轻人白内障的发生中起到了重要作用。

Objective：To observe the expression and investigate the clinical significance of Platelet derived growth factor-A （PDGF-A） in epithelial cells of age-related cataract lens. Methods： 70 cases of age-related cataract lens and 10 cases of transparent crystal were selected as the experimental group and control group, respectively. The expression of PDGF-A in epithelial cells were detected by SP immunehistochemistry staining. The quantitative analysis was performed by professional image software and the data were analyzed with standard statistical method. Results：Compared with the normal transparent lens, the expression level of PDGF-A was higher in patients suffering from age related cataract. In addition, the number of positive cells went up with the level of the opacities of lens. There were significant differences in the percentage of positive cell in the anterior capsule between ARC group and control group （P 〈0.05）. Conclusion： （1）PDGF-A was highly expressed in the age-related cataract. （2）The abnormal expression of PDGF-A was related to the age-related cataract. There was a positive relationship between the expression level of PDGF-A and the lens opacities classification. （3） Over expression of PDGF-A probably played an important role in the formation of ARC curling on the younger.