miR-224在原发性肝癌患者血清中的表达及意义

Expression and Significance of miR-224 in Serum on Primary Hepatocellular Carcinoma

目的:原发性肝癌(primary hepatocellular carcinoma,PHC)作为常见的恶性程度极高的肿瘤,严重威胁着人类的生命。miR-224是近年来发现的一个肿瘤相关miRNA分子,在肿瘤的发生及发展过程中发挥着重要的作用。本研究通过测定原发性肝癌患者血清中miR-224的表达水平,探讨血清miR-224与原发性肝癌预后的关系。方法:采用实时荧光定量RT-PCR(Real-time RT-PCR)方法,分别检测40例原发性肝癌患者,20例慢性肝炎患者,20例慢性肝硬化患者及20例正常人的血清标本中miR-224的表达水平。分析血清miR-224的表达水平与AFP和MMP-9的相关性。结果:原发性肝癌患者血清miR-224的表达水平明显高于正常人、慢性肝炎和慢性肝硬化患者(P<0.05)。皮尔森相关分析结果显示原发性肝癌患者血清miR-224的表达与AFP和MMP-9呈正相关。血清miR-224低表达组术后复发/转移率显著低于高表达组,术后生存率则高于高表达组(P<0.01)。结论:miR-224在原发性肝癌患者的血清中呈高表达,其血清表达水平与原发性肝癌的临床预后密切相关。这提示我们,miR-224可能成为新的原发性肝癌检测标记物和潜在的原发性肝癌预后分子标志物。

Objective： As a common malignant tumor, the primary liver cancer （primary hepatocellular carcinoma, PHC） has seriously threatened the human life. Recently, miR-224 has been discovered as the relative micromas molecules of tumor that played an important role on the occurrence and development process of tumor. This research aims to investigate the expression of miR-224 in serum on primary hepatocellular carcinoma, as well as the relationship with the prognosis of primary hepatocellular carcinoma. Methods： The blood samples were collected from 40 patients with PHC, 20 patients with chronic hepatitis, 20 patients with chronic hepatic cirrhosis and 20 healthy people. The expression levels ofmiR-224 in the serum were measured by reverse transcription polymerase chain reaction. The correlations of miRNA-224 expression, traumatic injury and the prognosis of primary hepatocellular carcinoma were analyzed. Results： The expression level of miR-224 in patients with PHC was significantly higher than those of patients with benign diseases and healthy people （P〈0.05）. Pearson＇s correlation analysis showed that the expression of miR-224 in serum had a close correlation with AFP and MMP-9 （P〈0.05）. The recurrent/metastatic of PHC patients with a lower miR-224 expression level was lower than those of people with higher expression, while the survival time of PHC patients with a lower miR-224 expression level was longer than those of people with a higher level expression （P〈0.05）. Conclusion： The expression ofmiR-224 in serum is high and it is closely relative to the prognosis of PHC which could be the potential marker for PHC and the target for treatment.