摘要
目的:研究白细胞介素10(IL-10)基因对链脲佐菌素(STZ)诱导的糖尿病大鼠胰腺炎症浸润程度及胰腺组织中Bcl-2及Bax表达的影响。方法:建立链脲佐菌素性糖尿病模型,腺病毒介导的IL-10基因(Ad-mIL-10)腹腔注射。检测大鼠空腹血糖值;免疫组织化学法观察胰腺炎症浸润程度;TUNEL法检测胰岛细胞凋亡;免疫组化方法观察Ad-mIL-10对实验性糖尿病大鼠胰岛凋亡调控基因Bax和Bcl-2表达的影响。结果:Ad-mIL-10腹腔注射糖尿病发病率低,平均血糖水平低,可以降低胰腺炎症浸润程度,减少胰岛细胞凋亡。给予Ad-mIL-10后大鼠Bax基因的表达明显下降,Bcl-2与Bax的比值明显增加。结论:IL-10基因对实验性糖尿病大鼠有降血糖作用,减少胰岛细胞凋亡,与调节Bcl-2与Bax基因的表达有关。
Objective： To investigate the effect of adenovirus mediated interleukin-10 gene on streptozotocin （STZ）-induced type 1 diabetic mellitus （T1DM） in SD rats, and to investigate the expression of Bcl-2 and Bax. Methods： Forty male SD mice aged from four to five weeks were randomly divided into 4 groups. Group 1 （vacuity group） was without special disposal; Group 2 was diabetes mellitus control goup; Group 3 received the intraperitoneal injection of adenovirus vector mediated empty green fluorescence protein （Ad-eGFP）; Group 4 received intraperitoneal injection ofadenovirus vector mediated IL-10 gene （0.2 mL）. Group2, 3 and 4 received intraperitoneal injection of stz 50 mg/kg in order to induce diabetic mellitus of SD mice. Pancreatic histology was measured for determination of insulitis grades. Apoptosis was measured by using a TUNEL assay. Expressions of Bcl-2 and Bax were estimated by immunohistochemistry analysis. Results： Compared with that in the control group, Ad-mlL-10 group had lower incidence in diabetes and lower degree of average blood glucose; Pancrea pathology showed that inflammatory infiltration was lighter and beta-cell apoptosis decreased. Decreased expression of Bax and incresed rate of Bcl-2/Bax was observed in the Ad-mlL-10 group. Conclusions： IL-10 gene therapie is able to decreasethe average blood glucose and exhibits anti-apoptotic ability. The expressions of Bax and Bcl-2 in the pancreas showed positive correlation with apoptosis.
出处
《现代生物医学进展》
CAS
2014年第4期671-675,共5页
Progress in Modern Biomedicine
基金
广东省自然科学基金项目(S2012040006330)
广东省科技计划项目(2012B031800016)
