Fascin蛋白在软骨肉瘤中的表达及其临床意义

Expression of Fascin and its Clinical Significance in Chondrosarcoma

目的:分析Fascin蛋白在软骨肉瘤组织中的表达及其与软骨肉瘤临床病理因素的关系。方法:应用免疫组化EnlivionTM法分别检测和比较70例软骨肉瘤和20例软骨瘤中Fascin蛋白的表达情况。结果:(1)Fascin在软骨肉瘤组和软骨瘤组中的阳性表达率分别为64.29%和5.00%,两组相比差异有统计学意义(X2=21.88,P=0.00);(2)Fascin蛋白的表达与患者的年龄、性别无显著相关性(X2=0.37,P=0.54;X2=0.29,P=0.59);Fascin蛋白在肺转移组中的表达为76.32%,显著高于无肺转移组(50.00%),两组相比差异有统计学意义(X2=5.24,P=0.02);Fascin蛋白在间叶性软骨肉瘤、透明细胞软骨肉瘤的中表达分别与普通型软骨肉瘤相比,无统计学意义(P>0.05),但去分化软骨肉瘤与普通型软骨肉瘤相比,有统计学意义(P<0.05);Fascin在普通型软骨肉瘤的Ⅰ-Ⅲ级中的阳性表达率分别为11.11%、61.90%、75.00%,三者之间的差异有统计学意义(P<0.05)。结论:Fascin参与了软骨肉瘤恶变的过程,其高表达与软骨肉瘤的分化和转移有关。

Objective： To analyze the expression of fascin protein in chondrosarcoma and relationship with clinicopathological factors. Methods： The expression of fascin was detected by immunohistochemisty （Enlivion TM） in 70 cases of chondrosarcoma and 20 cases of chondroma. Results： （1）The expression rate of fascin protein in chondrosarcoma and chondroma were respectively 64.29% and 5.00%, which was significantly higher in chondrosarcoma （X2=- 21.88, P〈0.00）; （2）The expression of fascin protein was not significantly related to age or gender （X2= 0.37, P=0.54; X2=0.29, P=0.59）; the expression rate of fascin was 76.32% in the chondrosarcoma with pulmonary metastasis, which was higher than that in the chondrosarcoma without pulmonary metastasis （50.00%）（X2=5.24, P=0.02）; no significant difference of fascin expression was found between conventional chondrosarcoma and mesenchymal chondrosarcoma or clear cell chondrosarcoma（P〉0.05）, but there was statistical difference between dedifferentiated chondrosarcoma and conventional chondrosarcoma （P〈0.05）. The expression of fascin in grade I -III of conventional chondrosarcoma were respectivelyl 1.11%, 61.90%, 75.00%, and there was statistical difference among them （P〈0.05）. Conclusion： Fascin took part in the process of canceration of chondrosarcoma and was related to the matastasis and differentiation of chondrosarcoma.