醛固酮瘤中多巴胺D2受体表达及甲基化研究

Expression and methylation of dopamine receptor D2 in aldosterone-producing adenoma

收集15例醛固酮瘤组织和3例正常肾上腺皮质组织，利用Real-timePCR和甲基化特异性PCR法分析多巴胺D2受体（Dopamine receptorD2，DRD2）的表达和甲基化情况。发现DRD2在病例组表达下调，DRD2启动子区域的甲基化部分参与醛固酮瘤该基因低表达的发生。

Real-time polymerase chain reaction（PCR） and methylation-specific PCR were used to test the expression of dopamine receptor D2 （DRD2） and the level of DNA methylation by screening 15 cases of aldosterone- producing adenoma（APA） and 3 normal adrenal cortex samples, mRNA level of DRD2 in APA was significantly lower than that in normal adrenal cortex. The methylated DRD2 plays a partial role in the expression of APA.