感染泡球蚴大鼠经门静脉灌注化疗栓塞后肝组织的病理变化

Pathological Changes of after Trans-Portal Vein Chemoembolization Echinococcus multilocularis in the Liver of Infected Rats

目的观察经门静脉途径灌注阿苯达唑脂质体和碘化油混悬液后大鼠肝泡状棘球蚴病的病理形态学变化过程,探讨其治疗效果。方法将20只感染泡球蚴的Wistar大鼠分为治疗组(19只)和感染对照组(1只),治疗组大鼠经门静脉穿刺灌注阿苯达唑脂质体和超液态碘化油混悬液0.2 ml,分别于治疗后4、7和10 d处死大鼠6、5和5只,取各组大鼠泡球蚴组织,HE染色和甲苯胺蓝染色进行病理学观察。结果感染对照组大鼠泡球蚴组织结构正常,治疗组大鼠经治疗后4 d,泡球蚴组织结构基本正常。7 d以变性改变为主,10 d以坏死改变为主。7和10 d均可见脂质体和碘油颗粒大量在泡球蚴组织内沉积,并引起肝泡球蚴组织结构破坏,囊泡塌陷,生发层、角质层结构退变。结论门静脉途径介入治疗肝泡球蚴病可能是一种有效的治疗技术方法。

Objective To observe the morphological change in the pathological process of Echinococcus multilocularis in liver of infected rats after treatment with liposome entrapped albendazole and iodized oil suspension through portal vein, and investigate its efficacy against hepatic alveolar echinococcosis. Methods One Echinococcus multilocularis- iniected rat served as control. Another 19 infected rats were infused with 0.2ml liposome entrapped albendazole and iodized oil suspension through portal vein. At 4, 7 and 10 d after treatment, the rats were sacrificed for further observation. The E. multilocularis metacestodes were collected, and the pathological change of alveolar hydatid was observed with hematoxylin- eosin staining and toluidine blue staining. Results In four days post treatment, E. multilocularis metacestodes were normal. On the 7th day and 10th day post treatment, the major pathological change was degeneration and necrosis, respectively. A great quantity of liposomes and iodized oil deposited in the metacestodes, and caused breakdown of E. multilocularis tissue, collapse of cysts, cataplasia of germinal layer and laminated layer. Conclusion Interventional therapy trans-hepatic vein may be an effective way to treat hepatic alveolar echinococcosis.