PaLCuCNV和TYLCCNV复合侵染引起更严重的番茄黄化曲叶病

The More Severe Tomato Yellow Leaf Curl Disease is Caused by Co-infection of PaLCuCNV and TYLCCNV

从四川攀枝花市田问采集36份表现严重矮化、黄化和曲叶症状的番茄病株样本，利用双生病毒简并引物PA／PB从所有样本中均扩增得到约500bp的片段，经全序列测定及分析，检测出中国番木瓜曲叶病毒（Papaya彪日厂curl Chinavirus，PaLCuCNV）和中国番茄黄化曲叶病毒（Tomatoyellow leaf curl Chinavirus，TYLCCNV），这两种双生病毒的复合侵染率达97．2％。系统进化分析表明，这两种双生病毒分别与已报道的PaLCuCNV河南番茄分离物（PaLCuCNV-[HeNZMI]）及TYLCCNV云南元谋烟草分离物（TYLCCNV-[Y295]）的核苷酸序列相似性最高，分别为99．1％和97．9％。检测发现，所有分离物均伴随有卫星DNAp分子，全序列测定表明所得9个DNAp分子均为TYLCCNV的卫星TYLCCNB，且与其四川番茄分离物（TYLCCNB．[SC65]）的核苷酸序列相似性最高，为87．7％～94．5％。本文首次报道PaLCuCNV与TYLCCNV／TYLCCNB病害复合体复合侵染番茄引起更严重的番茄黄化曲叶病。

Thirty-six tomato samples with severe stunting, yellowing and leaf-curling symptoms were collected in Panzhihua, Sichuan Province. Fragments of about 500 bp were amplified from all samples using the begomoviruses degenerate primers PA and PB. The complete genome sequence analysis showed that the diseased tomato samples were infected by Papaya leaf curl China virus （PaLCuCNV） and Tomato yellow leaf curl China virus （TYLCCNV） and 97.2% samples were infected with both viruses. Sequence analysis showed that PaLCuCNV had the highest sequence identity （99.1%） with PaLCuCNV- [HeNZMI] isolated from tomato in Henan, and TYLCCNV shared the highest sequence identity （97.9%） with TYLCCNV- [Y295] isolated from tobacco in Yuanmou, Yunnan. In addition, all isolates were found to be associated with DNA 13 molecules. Nine complete DNA [3 sequences were determined and showedthe highest sequence identity with DNA β of TYLCCNV （TYLCCNB-[SC65] ）, being ranged from 87.7% to 94.5%. This is the first report that tomato yellow leaf curl disease was caused by the co-infection of PaLCuCNV and TYLCCNV/TYLCCNB.