壳聚糖天然高分子衍生物促进大鼠皮肤愈合

Promotion of Rat Skin Healing by Using Natural Polymer Chitosan Derivatives

本文通过动物实验,观察N-果糖修饰的壳聚糖季铵盐衍生物对大鼠皮肤伤口愈合的效果。实验用SD大鼠40只,随机分8组。其中4组为实验组,4组为对照组。沿背部脊柱旁皮肤建立1.50cm×2.00cm×0.16cm全层皮肤切除伤口模型,实验组用N-果糖修饰的壳聚糖季铵盐衍生物软膏敷伤口,对照组用无菌医用凡士林进行处理。每日换药2次,观察各组伤口愈合。在3、7、10、15d之后观察各组创面愈合情况,创口病理观察。结果显示伤口愈合时间在实验组和对照组之间差异有统计学意义(P<0.05);创口愈合面积和容积愈合程度两组之间差异有统计学意义(P<0.05)。综上所述,N-果糖修饰的壳聚糖季铵盐衍生物对皮肤没有毒副作用,对皮肤愈合有促进作用,适宜作为皮肤修复材料,是医用敷料的理想原料。

We in the present study observed the effect of N-fructose modified chitosan quaternary ammonium derivativeson on rat skin wound healing through animal experiments. Forty rats were randomly divided into eight groups （5 in each group）. Four groups among the all 8 groups were the experimental groups, while the other 4 groups were the control groups. Next to the skin along the back of the spine, 1.50 cm×2.00 crux 0.16 cm full-thickness skin was cut to make an excision wound model for every rat. Those in the experimental groups were treated with the N-fructose-modified chitosan quaternary ammonium derivatives ointment dressing the wound, while those in the control groups with sterile medical vaseline processing. We dressed the wounds twice a day to observe the wound healing of all rats in different groups. We then observed the wound healing and wound pathology after 3, 7, 10, 15 days respectively in different groups. Results showed significant differences of the time of wound healing, area of wound healing and volume of wound healing between the experimental groups and control groups （P〈0.05）. It can be well concluded that N-fructose-modified chitosan quaternary ammonium derivatives does not harm the skin, but could promote skin healing, so that they could be suitable skin repair materials and ideal raw materials for medical dressing.