摘要
目的:研究不同管径TiO2纳米管形貌对软组织愈合的影响。方法:用阳极氧化法并以5、20、25 V电压分别在钛种植钉表面制备不同管径的TiO2纳米管涂层(NT-5、NT-20、NT-25),以抛光纯钛种植钉为对照组(PT);场发射扫描电镜(FE-SEM)观察试样表面形貌,测量去离子水、二碘甲烷和乙二醇在试样表面的接触角及其表面能后,将各组种植钉植入4只杂种犬的双侧下颌;分别于植入后1、7、14、28 d时各处死1只动物并取材,组织学观察种植体-软组织界面的愈合情况;实时定量PCR检测植体周围组织中FGF-2 mRNA水平表达。结果:5、20、25 V电压可制备管径约30、100、130 nm的TiO2纳米管涂层,随着管径增大试样表面接触角变小,表面能增高;NT-25组的组织修复改建完成最早,其次为NT-20组,NT-5组与PT组未见明显差异;FGF-2 mRNA的表达在1、7 d时NT-25组最高(P<0.05),14 d时开始降低,28 d时各组间比较P>0.05。结论:管径为130 nm的纳米管比较小管径的种植体表面形貌有利于软组织的早期修复和改建。
AIM： TO study the influence of TiO2 with different nanotube diameters on soft tissue healing. METHODS： TiO2 nanotube with different diameters were fabricated on polished pure titanium implants（PT） by anod- ization at 5V,20V and 25V （ NT-5, NT-20 and NT-25） respectively with PT as the control The surface topograph was observed by field emission scanning electron microscope（FE-SEM）. Contract angle was measured with deionized water, methyl iodide and formamide respectively . The interface energy was calculated. Then, the implants were im- planted into bilateral mandibular of 4 dogs. One dog was sacrificed 1 d, 7d, 14d and 28d after implantation respective- ly, the healing interface between implants and soft tissue was abserved by histological method, FGF-2 content of the surrounding soft tissue was examined by real time PCR. RESULTS： Anodization at 5V, 20V, 25V voltage produced 30 nm, 100 nm and 130 nm nanotube coating on the TiO2 implants. With the increase of nanotube diameter, the surface contact angle decreased, surface energy increased. Complete tissue healing was observed in the sampls of NT-25 group, followed by those of NT-20 group. No significant difference was observed between NT-5 and PT groups. High- est FGF-2 expression was found at the 1 st and 7th day in the soft tissue of NT-25 group （ vs other groups, P 〈 0.05）,FGF-2 expression decreased at 14th day, and 28th day there was no statistic difference among the groups（ P 〉0.05 ）. CONCLUSION： TiO2 implant surface with nanotube of 130 nm in diameter is more favorable for soft tissue repair than that with smaller diameter.
出处
《牙体牙髓牙周病学杂志》
CAS
北大核心
2014年第2期95-100,共6页
Chinese Journal of Conservative Dentistry
基金
国家自然科学基金资助项目(81070862)
