快速溶剂萃取-GC/MS法测定全血中沙蚕毒素

GC /MS determination of nereistoxin in whole blood with accelerated solvent extraction

目的采用快速溶剂萃取-GC／MS分析方法检验血液中杀虫双代谢产物沙蚕毒素。方法样本用硅藻土、中性三氧化二铝处理，用乙酸乙酯：环己烷：丙酮（2：2：1）进行萃取，GC／MS方法进行测定，并考察实验最佳条件。结果沙蚕毒素在50～2000ng／mL范围内线性关系良好，相关系数为0．999，检测限为15ng／mL，平均回收率为98．22％，相对标准偏差为4．6％。结论速溶剂萃取-GC／MS分析方法简便，快速，准确、灵敏度高，可在血中沙蚕毒素检验中选用。

Objective To establish a method for determination of nereistoxin in human plasma by accelerated solvent extraction gas-chromatography. Methods We extracted nereistoxin from plasma by ethyl acetate ： cyclohenane ： acetone （2 ： 2 ： 1 ） and detected it with GC/MS. Results The linear range of nereistoxin was 50 - 2000ng/mL（R2 = 0. 999）, the minimal detection limits （LOD） was 15 ng/mL, and the average recoveries of the nereistoxin was 98.22%, respectively. Conclusion The method has high sensitivity and good reproducibility without the endogenous interference. The result is accurate and reliable with a wide linear range. It can be used in forensic toxicological analysis.