摘要
目的:观察高效抗逆转录病毒疗法(HAART)治疗24周后获得性免疫缺陷综合征(AIDS)患者辅助性17(Th17)细胞和调节性T(Treg)细胞表达水平的变化。方法选取HAART治疗的45例AIDS患者,将其按CD4+T细胞计数分成1~100 cells/μl、101~200 cells/μl和201~350 cells/μl三组,于抗病毒前和抗病毒24周后采集静脉血,运用流式细胞仪检测外周血中CD4+T细胞、Th17和Treg细胞的表达水平,并比较分析其相互关系。结果 AIDS患者治疗24周时Th17细胞在CD4+T细胞中的表达频率为(1.91±0.84)%,而治疗前为(1.31±0.45)%,差异有统计学意义(t=3.67, P<0.05); Treg细胞表达频率在治疗24周时和治疗前分别为(5.97±0.93)%和(8.32±1.12)%,差异有统计学意义(t=3.32,P<0.05)。治疗24周时,Th17细胞明显升高,CD4+T细胞基数为201~350 cells/μl患者组其Th17细胞表达频率显著高于101~200 cells/μl和1~100 cells/μl组患者(t分别=2.68、4.51,P均<0.05)。 Treg细胞表达频率经治疗后显著下降,CD4+T细胞基数为201~350 cells/μl组患者Treg细胞表达频率下调程度显著高于101~200 cells/μl和1~100 cells/μl组患者(t分别=2.47、3.89,P均<0.05)。治疗前CD4+T细胞数与Th17表达的百分率呈正相关(r=0.48, P<0.05),与Treg表达的百分率呈负相关(r=-0.30,P<0.05)。结论 AIDS患者Th17表达水平下降,Treg表达则上升;HAART治疗能有效地上调Th17表达频率,下调Treg细胞水平;CD4+T细胞基数高的患者较基数低的患者对抗病毒治疗其免疫反应更有效,提示Th17和Treg细胞表达频率可作为HAART治疗有效性的一项判断依据。
Objective To observe the expression levels of Th17 and Treg cells of acquired immune deficiency syn-drome(AIDS) suffered patients after 24 weeks highly active antiretroviral treatment(HAART). Methods According to the CD4+T cell amounts , 45 AIDS patients received HAART treatment were divided into three groups. Flow cytometry technol-ogy was used to detect the CD4+T Cells count, Treg and Th17 cells in peripheral blood at the time of pre-therapy and 24 weeks. Results The ratio of Th17 cells in AIDS patients was (1.91±0.84)%in 24 weeks, and was (1.31±0.45)% at the time of pre-therapy, the difference was significant(t=3.67, P〈0.05). The expression level of Treg was (5.97±0.93)%and (8.32±1.12) % at the time of 24 weeks treatment and pre-therapy with statistical significance (t=3.32,P〈0.05). Th17 cells expression level was raised after 24 weeks, and which of CD4+T cells for 201~350 cells/μl group showed a remarkable decline than those of CD4+T cells for 101~200 cells/μl and 1~100 cells/μl groups (t=2.68,4.51,P〈0.05).Treg cells ex-pression level were down-regulated after 24 weeks treatment, and the dropped levels with of CD4+ T cells for 201~350cells/μl group was higher than those of two other groups(t=2.47,3.89,P〈0.05). Th17 cells was significantly positively related to the percentage of CD4+T cells count(r=0.48, P〈0.05);the correlation of Treg expression and CD4+T Cells count was significantly negative(r=-0.30, P〈0.05) at the time of pre-therapy. Conclusions The expression level of Th17 cells decreased,while Treg cells was increased in AIDS patients; HAART treatment could upregulated Th17 cells level and downregulated Treg cells. The patients with higher baseline of CD4+T cells has higher immune reaction effective than that of the lower baseline CD4+T cells. The Th17 and Treg cells are likely to be the effective indexes to observe HAART progress.
出处
《全科医学临床与教育》
2014年第1期26-29,共4页
Clinical Education of General Practice
基金
绍兴市重点科技计划项目(2011A23016)