高效液相色谱-蒸发光散射法检测芪白平肺胶囊中3种人参皂苷含量

Determination of Three Ginsenosides in Qibai Pingfei Capsules by High-Performance Liquid Chromatography-Evaporative Light-Scattering Detector

目的 采用高效液相色谱仪-蒸发光散射检测器（high performance liquid chromatography-evaporative light scattering detector,HPLC-ELSD）建立芪白平肺胶囊中人参皂苷Rg1、Re和Rb1的含量测定方法.方法采用色谱柱为Hypersil ODS XB-C8柱（4.6 mm×250 mm,5 μm）和Hypersil ODS XB-C18柱（4.6 mm×250 mm,5 μm）,以乙腈为流动相A、以水为流动相B进行梯度洗脱;洗脱程序（0～40 min,A 19%;40～55 min,A 19%～40%）,流速为1.2 ml/min,柱温为30 ℃.ELSD参数优化：漂移管温度分别设为50、70、90、110 ℃,载气流量分别为1.0、1.2、1.4、1.6 L/min.结果 通过对比研究,最终采用Hypersil ODS XB-C8柱作为分析柱,漂移管温度为90 ℃,载气流量为1.2 L/min时,3种人参皂苷的含量检测结果最佳.人参皂苷Rg1、Re和Rb1的加样回收率分别为98.3%、99.1%、101.8%,RSD分别为0.67%、1.03%、1.22%.结论 所选择的色谱柱和优化的ELSD参数可准确测定芪白平肺胶囊中3种人参皂苷的含量,且重现性好.

Objective To establish a method for determining the content of ginsenosides Rg1, Re, and Rb1 in Qibai Pingfei Capsules by high-performance liquid chromatography-evaporative light-scattering detector （HPLC-ELSD）. Methods HPLC was performed on a Hypersil ODS XB-C8 column （4.6 mm × 250 mm, 5 μm） or Hypersil ODS XB-C18 column （4.6 mm ×250 mm, 5μm） with acetonitrile and water as mobile phases A and B for gradient elution （0-40 min, A 19%; 40-55 min, A 19%-40%） at a flow rate of 1.2 ml/min and a column temperature of 30℃. For ELSD parameters, the drift tube temperature was set at 50℃, 7 0℃, 90 ℃, or 110 ℃, and the carrier gas flow rate was set at 1.0 L/min, 1.2 L/rain, 1.4 L/rain, or 1.6 L/min. Results By contrast, the determination of three ginsenosides in Qibai Pingfei Capsules had the optimal results when Hypersil ODS XB-C8 column was used as the analysis column, the drift tube temperature was set at 90 ℃, and the carrier gas flow rate was set at 1.2 L/min. The average recoveries of ginsenosides Rg], Re, and Rbl were 98. 3% （RSD=0. 67%）, 99.1% （RSD=I. 03%）, and 101.8%（RSD= 1.22%）, respectively. Conclusion The HPLC-ELSD method using selected chromatographic column and optimized ELSD parameters has high accuracy and good repeatability in determining the content of ginsenosides Rgl , Re, and Rb1 in Qibai Pingfei Capsules.