中耳胆脂瘤上皮细胞DNA的特征

DNA character of the cholesteatoma

目的 :采用流式细胞术分析胆脂瘤上皮细胞的 DNA特征 ,探讨胆脂瘤的病理学属性。方法 :利用机械捣碎法将新鲜的胆脂瘤和外耳道上皮制成单细胞悬液 ;采用 PI法 ,对 2 5例胆脂瘤上皮和 10例正常外耳道细胞上皮的 DNA进行流式细胞术分析。结果 :胆脂瘤上皮细胞和外耳道上皮细胞的核型均为二倍体 ,未见异倍体。胆脂瘤上皮细胞 S期细胞数 (10 .9± 2 .32 ) %明显高于外耳道上皮 (7.31± 2 .82 ) %。结论 :虽然胆脂瘤破坏力强 ,易复发 ,但从染色体水平看来 ,仍提示为一种炎性病变 ,未见有恶变倾向。

Objective:To compare the DNA character with in cholesteatoma tissues with that in normal skin.Method:DNA content within 25 cholesteatoma tissues and 10 normal postauricular skin was measured with flow cytometry.Result:The content of cholesteatoma tissues and postauricular skin specimens are a normal euploid.The cell number during S phase of cholesteatoma( 10.9 ± 2.32 )% was higher than that in normal skin( 7.31 ± 2.82 )%.Conclusion:Although the cholesteatoma has a very high proliferating character,the result suggest it isn′t a malignant tumor.