摘要
AIM: To investigate the cytotoxicity, anti-inflammatory activity, and action mechanism of root bark extracts of Acanthopanax henryi. METHOD: The hot methanol extract of the root bark of A. henryi was subjected to XAD-4 column chromatography eluting with a gradient of methanol in water. The cytotoxicity and anti-inflammatory effects of the MeOH fractions were evaluated on the inhibition on lipopolysaccharide(LPS)-induced nitric oxide, prostaglandin E2, interleukin-1β, and interleukin-6 production in RAW 264.7 macrophages. RESULTS: The 80% MeOH fraction was a better inhibitor of LPS-induced NO, PGE2, IL-1β, and IL-6 production, and expression of inducible nitric oxide synthase(iNOS) at the protein levels in a concentration-dependent manner. CONCLUSION: The 80% MeOH fraction of A. henryi root bark has significant anti-inflammatory activity. This provides a pharmacological basis for clinical application for the treatment of inflammation.
AIM： To investigate the cytotoxicity, anti-inflammatory activity, and action mechanism of root bark extracts of Acanthopanax henryi. METHOD： The hot methanol extract of the root bark ofA. henryi was subjected to XAD-4 column chromatography eluting with a gradient of methanol in water. The cytotoxicity and anti-inflammatory effects of the MeOH fractions were evaluated on the inhibi- tion on lipopolysaccharide （LPS）-induced nitric oxide, prostaglandin E2, interleukin-1β, and interleukin-6 production in RAW 264.7 macrophages. RESULTS： The 80% MeOH fraction was a better inhibitor of LPS-induced NO, PGE2, IL-1β, and IL-6 production, and expression of inducible nitric oxide synthase （iNOS） at the protein levels in a concentration-dependent manner. CONCLUSION： The 80% MeOH fraction ofA. henryi root bark has significant anti-inflammatory activity. This provides a phar- macological basis for clinical application for the treatment of inflammation.
基金
supported by the PhD Program Open Foundation of the Food and Drug Administration of Korea
Hunan Provincial Natural Science Foundation(No.11JJ2042)
the Science of Pharmaceutical Analysis of Twelfth Five-Year Key Discipline Projects of Hunan University of Chinese Medicine
Science of Chinese Materia Medica of Twelfth Five-Year Key Discipline Projects of Hunan Province
