Cloning and Expression of C4B Gene in Siji Goose

四季鹅C4B基因的克隆及其表达(英文)

[Objective] This study aimed to clone C4B gene in Siji goose and detect its expression level in different tissues. [Method] cDNA sequence of C4B gene was cloned with RACE-PCR method. Amino acid sequences in multiple species were aligned in GenBank, and a phylogenetic tree was constructed for homology analysis. [Result] C4B gene in Siji goose shared relatively high homology with chicken and quail; Siji goose C4B gene was expressed highly in liver and lung of adult geese and expressed lowly in epididymis, seminiferous duct, brain, kidney, testis, heart, oviduct and smal intestine. [Conclusion] In the present study, mRNA expression lev-el of C4B gene in different tissues and organs of Siji goose was determined by flu-orescence quantitative PCR, which provided basis for rapid diagnosis of specific an-imal diseases.

[目的]克隆四季鹅的C4B基因,并检测其在鹅体内的表达情况。[方法]应用RACEPCR的方法克隆C4B基因的cDNA序列,登录GenBank进行多物种蛋白氨基酸序列比对并构建进化树,以分析同源性。[结果]四季鹅C4B与鸡、鹌鹑2种禽类的同源性较高;在成体鹅中C4B主要在肝、肺中呈现高水平表达,在附睾、输精管、脑、肾、睾丸、心、输卵管、小肠中呈低表达。[结论]该试验应用荧光定量PCR的方法测定了C4B基因在正常状态下四季鹅不同组织、器官中mRNA的表达量,为快速诊断某些疾病提供依据。