槲皮素对NB4细胞株p53及蛋白的影响

The Effect of Quercetin Targeted p53 Gene and Protein Expression in NB4 Cell Lines

目的研究治疗白血病的有效药物。 方法将槲皮素作用于培养的NB4细胞株 ,应用RT -PCR及Westernblot方法 ,研究不同浓度槲皮素 (30、6 0、90 μmol/L)诱导细胞 2 4、48、72、96、12 0h后 ,急性早幼粒细胞白血病NB4细胞株中突变型p5 3基因及蛋白表达的情况。 结果证实其在 30～ 90 μmol/L浓度时对突变型p5 3基因无影响 ,而对突变型p5 3蛋白的表达有抑制作用。 结论本研究为槲皮素应用于急性早幼粒细胞白血病的研究提供了有效手段。

Objective In order to study the value of drug treatment for leukemia, quercetin was delivered to the cultured NB4 cell line. Methods Using RT-PCR and western blot, we studied the expression of mutant p53 gene and protein after inducing 24, 48, 72, 96 and 120h by the quercetin with different concentrations (30, 60, 90μmol/L). Results We verified the results that quercetin with concentration of 30 ～ 90μmol/L could successfully inhibit the expression of mutant p53 protein, but the mutant p53 gene did not have. Conclusion That data suggested that quercetin would be an effective method for the therapy of leukemia.