摘要
目的建立兔眼房水中包甲素类似物ATST含量的高效液相色谱 (HPLC)测定法。 方法以奥美拉唑(OMZ)为内标 ,流动相组成为甲醇 - 1%三乙胺 (5 7∶43) ,紫外检测波长为 2 35nm。 结果兔眼房水样品中药物浓度在2 .0 5 6～ 41.12 μg/ml范围内线性关系良好 ,r =0 .9997,平均回收率为 94.5 8% ,日内和日间标准偏差 (RSD)分别小于5 %和 10 % (n =5 )。 结论本方法稳定、可靠 ,可用于ATST的药动学研究。
Objective A HPLC method was established for determination of the content of ATST in the aqueous humor of rabbits. Methods The mobile phase was consisting of methanol-1% triethylamine(57∶43) and omeprazole (OMZ) as internal standard.The detection was carried out with an ultraviolet detector operated at 235nm. Results There was linearity over the range of 2.056 ～ 41.12 μg/ml in the humor aquosus,r=0.9997.The average recovery of ATST was 94.58%.Intra-day and inter-day RSD were less than 5% and 10%(n=5),respectively. Conclusion The method is reliable.It can be used for the study on the pharmacokinetics of ATST.
出处
《上海第二医科大学学报》
CSCD
2001年第1期20-21,共2页
Acta Universitatis Medicinalis Secondae Shanghai
基金
上海市教委重点学科发展基金资助(S99209)
