肠易激综合征患者5-羟色胺含量的变化

The Alteration of 5-Hydroxytryptamine Content in the Patients with Irritable Bowel Syndrome

目的探讨 5 -羟色胺 (5 -HT)与肠易激综合征 (irritablebowelsyndrome,IBS)发病的关系。 方法应用荧光分光光度法测定IBS患者和对照组血清 5HT、游离色氨酸 (TP)和总TP含量。应用高效液相色谱仪测定乙状结肠粘膜中 5 -HT的含量。 结果与对照组比较 ,腹泻型血清 5 -HT浓度 (496 .2 7± 12 8.2 2ng/ml)显著升高 (P <0 .0 5 )。两种类型乙状结肠粘膜 5 -HT含量 (1877.6 7± 6 5 5 .0 0ng/g ,1899.5 5± 817.89ng/g)均显著高于对照组 (12 0 2 .6 9± 5 12 .6 9ng/g) (P <0 .0 5 )。 结论 5 -HT浓度升高可能与IBS发病有关 ;IBS患者 5 -HT含量升高与血清中总TP或游离TP浓度无关。

Objective To study the relationship between 5-hydroxytryptamine (5-HT) and the patients with irritable bowel syndrome (IBS). Methods The contents of free tryptophan (TP), total TP, 5-HT in serum and 5-HT in sigmoid colon mucosa specimens were detected by spectrofluorophotometry and high-performance liquid chromatograph (HPLC) with thin-layer electrochemical (EC) detection method in patients with irritable bowel syndrome (IBS) and healthy volunteers. Results The concentrations of 5-HT in the serum of diarrhea-predominant IBS, the contents of 5-HT in both diarrhea-predominant IBS and constipation-predominant IBS in sigmoid colon mucosa were significantly higher; the concentrations of free and total TP were similar to those of the control group. Conclusion The results suggest that higher level of 5-HT may be involved in the pathogenic mechanism. The results also suggest that the increased content of 5-HT in the patients with IBS is not related to total and free TP in serum.