RP-HPLC法测定不同北虫草子实体和培养基中腺苷和虫草素含量的比较

Analysis on Contents of Adenosine and Cordycepin in Cordyceps Sinensis and Its Culture Medium by RP-HPLC

目的:比较九种北虫草子实体以及3种培养基中腺苷和虫草素的含量。方法:超声水提法提取样品中腺苷和虫草素,用RP-HPLC法测定其含量。结果:九种北虫草子实体腺苷和虫草素含量都存在差异,腺苷含量为(1.054±0.259)-(2.931±0.356)mg·g-1;虫草素含量差异较大,为(0.435±0.016)-(4.981±0.212)mg·g-1。3种不同培养基中虫草素和腺苷也存在差异,其中3号培养基虫草素含量最高,达1.046 mg·g-1,1号培养基腺苷最高,为0.612 mg·g-1。结论:九种北虫草子实体腺苷和虫草素含量差异较大;三种培养基中腺苷和虫草素含量也存在差异。

Objective： To analyze the contents of adenosine and eordyeepin in nine kinds of Cordyeeps sinensis as well as three kinds of culture mediums. Methods： The adenosine and eordyeepin were extracted with ultrasonic methods, and the con- tents of them were determined by RP-HPLC. Results： There was difference among the contents of adenosine and cordycepin in nine kinds of CML. The contents of adenosine varied from （1.054＋0.259）mg·g-1 to （2.931±0.356）mg·g-1 and the contents of eordyeepin varied from（0.435±0.016）mg＇g-1 to （4.981±0.212） mg·g-1. There was also difference among the three kinds of culture mediums; the contents of eordyeep of culture medium No.3 was 1.046 mg·g-1, which was the highest; the content of adenosine of culture medium No.1 was 0.612 mg·g-1, which was the highest. Conclusion：There was difference among the contents of adenosine and cordycepin in nine kinds of CML as well as in three kinds of culture mediums.