Guillain-Barre综合征患者B淋巴细胞辅助受体CD21表达研究

Expression of co-receptor CD21 on peripheral B lymphocytes in patients with Guillian-Barre syndrome

目的本研究通过检测Guillain-Barre综合征(GBS)患者外周血B淋巴细胞辅助受体CD21的表达,探讨CD21与GBS及其严重程度之间的关系。方法利用流式细胞术检测GBS组(36例)和正常对照组(20例)CD21表达,按病程将GBS组分为急性期亚组与恢复期亚组,按病情轻重将GBS组分为轻症组和重症组,并分析CD21与疾病病程和严重程度之间的关系。结果与正常对照组(45.32±8.92)%比较,GBS组(73.48±7.63)%B淋巴细胞CD21表达显著升高,差异有统计学意义(P<0.01)。与恢复期GBS组(70.15±10.00)%比较,急性期GBS组(77.58±7.39)%B淋巴细胞CD21表达无明显差异(P>0.05)。与轻症GBS组(68.40±4.81%)比较,重症GBS组(75.98±7.90)%B淋巴细胞CD21无明显差异(P>0.05)。结论 CD21在GBS患者中表达显著升高,但与病程和病情严重程度无关。

Objective To explore the corelation between the expression of CD21 and the severity of GBS by detection of CD21 of B lymphocyte co-receptor in peripheral blood mononuclear cells（PBMNCs） of patients with GBS. Methods GBS group （36 cases）and healthy controls （HC） group（20 cases） were investigated. The expression of CD21 on peirpheral B lymphocytes were examined by flow cytometry. According to course of disease, GBS group was divided into acute subgroup and recovery subgroup. According to the severity of disease,GBS group was divided into mild group and severe group, Furthermore, the co- relation between the expression of CD21 and the course and severity of the disease were analyzed. Results Compared with HC group （45.32 ± 8.92 ） %, the CD21 increased significantly in GBS group （ 73.48 ± 7.63 %） （P〈 0.01 ）. Compared with recovery subgroup（70.15±10.00）%, the CD21 in the acute subgroup（77.58±7.39） % has no significant differences（P〈0.05）. Com- pared with mild group） （68.40±4.81） %, the CD21 in severe group（75.98±7.90）% had no significant differences（P〈0.05）. Conclusion CD21 expression in GBS significantly increases, but has no correlaiton with the the course and severity of the dis- ease.