MTT法体外检测癌细胞药敏试验及临床相关性研究

Anti-Cancer Drugs Sensitivity Testing in vitro by MTT Assay and its Correlation with Clinical Response

目的 研究MTT法检测癌细胞对抗癌药物的敏感性与临床个体化化疗的相关性。方法 取 5 7例癌症患者新鲜癌细胞 ,采用MTT法检测癌细胞对 8种常用抗癌药的敏感性 ,成功 46例 ,根据药敏结果对患者进行化疗 ,无敏感药物者按经验方案化疗 ,观察实验与临床结果的相关性。结果 药敏试验阳性者 2 5例 ,阴性者 2 1例。实验提示敏感药物化疗者的有效率为 76 .0 %(19/2 5 ) ,耐药者按经验方案化疗 ,其有效率仅为 2 8.6 % (6 /2 1) ,两者有非常显著性差异 ,P <0 .0 1。实验与临床符合率达 73 .9%(34/46 ) ;说明MTT法对癌症患者临床个体化化疗效果有预见价值。结论 MTT法体外癌细胞药敏试验可以指导临床个体化治疗。

Objective To study the anti cancer drugs sensitivity testing in vitro by the MTT assay and its correlation with clinical response.Methods Fifty seven patients with solid tumor were entered in this study.Chemosensitivity of fresh tumor cell was tested with the MTT assay for 8 anti cancer drugs.The MTT assay was successfuly performed in 46 of the 57 patients.These patients received chemotherapy on the basis of MTT assay results.The 25 patients of MTT sensitivity group were given chemotherapy according to results of drug sensitivity and the other 21 patients of MTT resistant group as controls were given chemotherapy according to clinical experience.Results Clinical responses were obtained in 19 of the 25 patients of chemosensitivity group(response rate:76.0%) and in 6 of the 21 patients of chemoresistant group(response rate:28.6%)( P <0.01).There was statistically significant difference.The overall coincident rate of clinical and testing resuts was 73.9%.Conclusion The chemosensitivity testing of tumor cells by MTT assay can be helpful to determine a individual chemotherapy protocol for solid tumour.