万乃洛韦抗鸭乙型肝炎病毒的实验研究

Experimental study on the effect of valaciclovir on antiduck hepatitis B virus

目的 观察万乃洛韦在体内抗鸭乙型肝炎病毒 (DHBV)的作用 ,为临床治疗乙型肝炎病人提供实验依据。方法 采用重庆麻鸭乙型肝炎动物模型 ,用万乃洛韦治疗 1个月 ,检测用药前后血清中的DHBVDNA及转氨酶、肝组织病理、肝组织中的DHBVDNA等。结果 万乃洛韦用药 1月能使血清DHBVDNA总体水平显著降低 (P <0 .0 5或P <0 .0 1) ,各剂量组血清DHBVDNA有阴转现象。肝脏病理检查及血清转氨酶检查发现 ,万乃洛韦对肝组织无明显影响 ,大剂量用药组ALT虽有轻度升高 ,但停药后即恢复正常。SouthernBlot检测肝组织内DHBVDNA ,提示该药能抑制病毒在肝内的复制 ,但不能彻底清除超螺旋DNA。

Objective we studied the effect of the Purine mucleoside Valaciclovir on anti duck hepatitis virus(DHBV) in vivo to provide an experimental basis for clinical treatment of patients with hepatitisB.Methods The Chongqing duck hepatitis B virus model was treated with Valaciclovir once a day for a month at the doses of 50mg.kg -1 、100mg.kg 1 、200mg.kg -1 of body weight per day. Serum DHBV DNA was detected four times in the course of the treatment,ALT and AST in serum and DHBV DNA in liver were detected simultaneously.Results Valaciclovir could signsificantly lower the serum DHBV DNA level. Serum ALT of several ducks in serum rose slightly during the treatment,but became normal after 1 week stopping Valaciclovir. Examination of DHBV DNA in liver with Southern Blot indicated Valaciclovir could inhibit DHBV DNA replication,but could not completely eliminate DHBV SC DNA.Conclusion The study confirms the safety and potent antihepaticviral activity of Valaciclovir in vivo.