霍乱毒素对金黄地鼠视网膜神经肽Y免疫反应节细胞再生的影响

The Effects of Cholera Toxin on the Regeneration of Neuropeptide Y-Immunoreactive Retinal Ganglion Cells in Adult Golden Hamster

【目的】研究NPY免疫反应 (IR)视网膜节细胞 (RGCs)能否再生及玻璃体内注射霍乱毒素 (CTx)或 /和玻璃体植入外周神经对其再生的影响。【方法】 16只成年金黄地鼠随机分 4组 ,每组 4只动物。切断视神经 (ON)并缝接坐骨神经 (at tachedgraft,AG)为再生对照组即AG组 ;ON切断并缝接坐骨神经后再于玻璃体内注射CTx或 /和植入小段坐骨神经 (SN)为实验组。荧光金逆行标记再生的RGCs结合NPY免疫荧光组化双标法 ,观察视网膜平铺片并记数。【结果】术后 4周 ,AG组每个视网膜NPY IR阳性再生RGCs平均数为 5 8± 2 2个 ,占再生RGCs总数的 3 36 % ;AG +CTx、AG +SN和AG +CTx +SN各实验组该类再生节细胞平均数依次为 (143± 47)、(12 5± 37)和 (4 37± 37)个 ,它们分别占再生RGCs总数的 (5 15±0 89) % ,(5 34± 0 37) %和 (8 11± 0 85 ) % ,与对照组的差异具统计学意义。【结论】成年哺乳动物NPY IR阳性RGCs能再生 ,玻璃体内注射CTx或 /和植入SN ,可能促进该类RGCs再生。

Objective To examine the regeneration of NPY immunoreactivity (IR) retinal ganglion cells (RGCs) and the effects of cholera toxin (CTx) injected or/and peripheral nerve implanted on regeneration of NPY IR RGCs. Methods 16 adult golden hamsters were ramdomly divided into 4 groups. Optic nerve (ON) was transacted and a segment of autologous sciatic nerve (attached graft, AG) was removed and sutured to the proximal stump of ON in regenerating control group (AG group). The animals in experimental groups were further treated with CTx injection or/and implantation of a short of segment sciatic nerve (SN) intravitrously. By using the retrograde labeling with fluorogold (FG) combined with fluorescent immunocytochemistry, the regenerated NPY IR RGCs were observed and counted under fluorescent microscope. Results At 4 weeks after surgery, the mean number of regenerated NPY IR RGCs per retina in AG group was 58±22 which constitutes (3 36±0 65)% of the total regenerated RGCs. In AG+CTx, AG+SN and AG+CTx+SN experimental groups, the mean numbers of regenerated NPY IR RGCs per retina were 143±47, 125±37 and 437±77 ordinally which constitute (5 15±0.89)%, (5 34±0 37)% and (8 11±0 85)% of the total regenerated RGCs respectively, which were increased significantly compared with those in AG group. Conclusion The results show that the axotomized NPY IR RGCs have the capability of regenerating their axons into the attached PNgraft, CTx and/or SN could enhance the regeneration of the NPY IR RGCs.