转基因植物表达质粒中SBR基因的序列分析

Sequence Analysis of SBR Gene in Expressional Plasmid of Transgenic Plant

【目的】对转基因植物表达质粒 pROP1中含SBR基因P1片段 ( 1 84～ 1 946bp)进行序列测定和分析。【方法】从E .coliDH5α中提取质粒 pROP1 ,用PCR测序试剂盒和DNA自动测序仪检测P1片段的序列 ,并与GenBank上的变形链球菌表面蛋白基因序列比较 ,检测其准确性。【结果】测定了P1片段 5′端 6 1 6个碱基序列和 3′端 46 6个碱基序列 ,其准确性达98%。【结论】PCR扩增的P1片段 5′端和 3′端的DNA序列与变形链球菌表面蛋白 pac基因唾液粘附区相一致 ,为转基因番茄防龋疫苗的研究提供了基础资料。

Objective To sequence P1 gene(184～1946 bp) including SBR gene in expressional plasmid of transgenic plant. Methods pROP1 plasmid was extracted from E. coli DH5α, P1 gene was sequenced by PCR sequence kit and DNA autosequencer and compared with surface protein gene of Streptococcus mutans in GenBank. Results 616 base pairs from 5′ end and 466 base pairs from 3′ end were sequenced, its accuracy is up to 98%. Conclusion The sequence of P1 gene's 5′ end and 3′ end is consistent with DNA sequence of SBR region of Streptococcus mutans surface protein pac gene and may provide useful informations to construct the transgenic plant anticaries vaccine.