PTCH1在胃食管连接部腺癌组织中的表达及临床意义

Expression of PTCH1 in Adenocarcinoma of Esophagogastric Junction and Its Clinical Significance

[目的]评价PTCH1在胃食管连接部腺癌(AEG)组织中的表达及意义。[方法]选择64例AEG组织、癌旁组织标本,应用RT-PCR和Western blot方法检测标本中PTCH1mRNA和蛋白的表达情况,并分析PTCH1蛋白的表达与AEG临床病理特征的关系。[结果]与癌旁组织相比,AEG组织中PTCH1 mRNA和蛋白的表达均明显下调,差异有统计学意义(P<0.05)。PTCH1蛋白表达与AEG患者性别、年龄、肿瘤大小和淋巴结转移无关,与肿瘤分化程度有关(P<0.05)。[结论]PTCH1在AEG组织中低表达可能与胃食管连接处腺癌的发生、发展相关。

[Purpose] To investigate the expression of PTCH1 in adenocarcinoma of esophagogas- tric junction （AEG） tissues and its clinical significance. [Methods] Sixty-four samples with AEG and AEG adjacent tissues were enrolled. The mRNA and protein of PTCH1 expressions were de- tected by RT-PCR and Western blot respectively. [Results] Compared to AEG adjacent tissues, PTCH1 mRNA and protein expression in AEG tissue samples were down-regulated with statisti- cally difference （P〈0.05）. The expression level of PTCH1 protein did not relate to gender,age ,tu- mor size and lymph node metastasis,but related to the differential grade of AEG （P〈0.05）. [Conclusions] The expression of PTCH1 is low in AEG;it might relate to carcinogenesis and progres- sion of AEG.